Tests

Test Name

Submission Requirements

Reference Range

Methodology

syphilis Syphilis Serology (RPR) 1 ml of serum or plasma Negative Rapid Plasma Reagin
17-Hydroxy- progesterone. 1ml serum. Morning specimen recommended. Male: 3.0-220 ng/dl Female:
Follicular: 20-100 ng/dl
Luteal: 100-500 ng/dl
Postmenopausal: <70 ng/dl
RIA
17-ketosteroids

Urine, Total



Urine, Fractionated
15 ml aliquot of well-mixed 24-hour urine. Place 1 tablet of boric acid in container prior to collection. Note 24 hour volume on requisition.

See Androsterone


Male: 7-20 mg/24 hrs.
Female: 5-15 mg/24 hrs.
Modified Zimmerman Reaction
3 Alpha Androstanediol Glucuronide 2 ml serum. Adult male: 2.6-16.0 ng/ml
Adult female: 0.60-8.1 ng/ml
Enzyme Digestion, Chromatography, RIA
5’ Nucleotidase

Serum
2 ml serum. 2-15 U/L Spectrophotometry
5-Hydroxyindole-acetic Acid (5-HIAA, Serotonin)

urine, Quantitative
10ml Aliquot of well mixed 24 hr. urine. Add 10 grams Boric Acid to container prior to collection. Note 24 hour volume on requisition. <9.0 mg/24 hr. HPLC or Immunoassay
5'Nucleotidase 2 ml of serum 2-15 U/L Spectrophotometry
ABO Antibody Titer

One full pink top tube. Do not use SST tube. Label test tube with patient full name. Not applicable Gel technology
ABO Group and Rh Type 1 pink top tube. (Do not use SST.)

Label tube with patient's full name and MR#
Not applicable Gel technology
Acetylcholine Receptor Antibody

Serum
1 ml serum. Collect in plain red top tube. Indicate if sample is from immunosuppressed patient. < 0.7 nmol Radio Binding Assay
Acid Fast Bacilli

Smear
Slide or specimen. No acid fast bacilli seen. Acid Fast stain
Acid Hemolysins

(Ham Test)
1 lavender top tube, plus 1 ml FROZEN serum 0% Lysis Acid Serum Lysis
Acid Phosphatase, Prostatic (PAP)

Serum
2 ml serum. Place on ice immediately after collection. FREEZE <2.5

mL
Chemiluminescence
Acute Hepatitis Panel See Test Profiles    
Adenovirus Antibody 1 ml serum Plain red top tube. Paired sera advised to detect rising titers. <1:8 Complement Fixation
Adrenocorticotrophic Hormone (ACTH)

Plasma
2 ml EDTA plasma. Draw in prechilled lavender top tube. Draw between 7am and 10am. Separate plasma and freeze in plastic vial. <70 pg/ml Chemilluminescence
Alanine Amino Transferase (ALT, SGPT) 1 ml serum (SST) 10-60 U/L Kinetic Assay
Albumin

Serum
1 ml serum (SST) Female: 3.6- 4.5 g/dl

Male: 3.5 -5.2 g/dl
Colorimetry endpoint

Timed endpoint
Alcohol, Ethyl

Serum
2 ml serum < 1.0 mg/dl Enzymatic
Aldolase

Serum
2 ml frozen serum. Separate serum and freeze in plastic vial. < 8.0 U/L Enzymatic
Aldosterone

Serum
2 ml serum. Draw in plain red top tube. Separate from cells within 30 minutes. FREEZE. Sodium intake 100-200 mEq/l

Supine: 2-9 ng/dl Upright: 3-35 ng/dl 2.3-21.0-mcg/24 hr. (Normal sodium intake)
Extraction, chromatography, RIA

Hydrolysis, extraction, RIA
Alkaline Phosphatase, Leukocyte (LAP) 1 green top tube.20-100 cell score 20-100 cell score Cytochemistry
Alkaline Phosphatase

Serum
1ml serum (SST) 26-88 U/L Kinetic
Allergy Testing

Rast Allergens
2ml serum (SST) per group of 6 allergens. REFRIGERATE. Specify number of and types of allergens on requisition. See report for interpretation

See addendum for list of Allergens available.
FEIA
Alpha-1-Antitrypsin

Serum, Total
1ml serum. Draw in plain red top tube. 83-199 mg/dl Nephelometry
Alpha-Fetoprotein (AFP)

Amniotic Fluid, Quantitative
2 ml amniotic fluid See report for interpretation. Chemiluminescence
Alpha-Fetoprotein (AFP)

Maternal Serum, Quantitative
2 ml serum See report for interpretation Chemiluminescence
Alpha-Fetoprotein (AFP)

Tumor Marker, serum
2 ml serum < 5.75 U/ml Chemiluminescence
Alpha-galactosidase A 3 Green top tubes (peripheral blood). DO NOT FREEZE. Submit to lab within 24 hours of collection. Submit with a completed genetic requisition form 0.016-0.20 U/L Fluorometric
Aluminum

Plasma
2 ml Heparinized Plasma-Dark blue top trace metal tube. Obtain KIT 493 from lab. Transfer plasma within 2 hours to aluminum-free plastic vial (included in KIT 493). 3-10 mcg/l Atomic Absorption
Aluminum

Serum
2mL serum- dark blue top tube. Obtain KIT 492 from lab. Transfer serum within 2 hours to aluminum free plastic vial (included in KIT 492). 3-10 mcg/l Atomic Absorption
Amikacin 2 ml serum. Collect in plain red top tube. Do not collect in SST Trough 0-10 us/ml

Peak 10-30 us/ml
EIA
Toxic > 35 us/ml
Amino Acid Fractionation

Plasma, Quantitative
2ml heparinized plasma (green top tube). Separate from cells immediately and place in plastic vial. FREEZE. Patient age and sex required. See report Chromatography (HPLC)
Amino Acid Fractionation

Urine. Quantitative
10ml aliquot of well-mixed 24-hour urine. Acidify to pH 3-5 with 6N HCI. Refrigerate while collecting. Note 24 hr. volume on requisition. Indicate patient’s age and sex. FREEZE. See report Chromatography (HPLC)
Amino Acid Screen

Plasma
3-5ml heparinized plasma (green top tube). FREEZE plasma in plastic vial. No Abnormal pattern detected. Chromatography (HPLC)
Amino Acid Screen

Urine
10ml frozen random urine. No abnormal pattern detected. Chromatography (HPLC)
Aminolevulinic Acid (ALA) 2ml aliquot of refrigerated well mixed 24 hr. urine. FREEZE. Protect from light by wrapping with aluminum foil. Acidify aliquot with 2 drops 6N HCL. Record total volume on Requisition. Do not acidify if requested in combination with urine lead. 6.4 mg/24hrs or less. Colorimetric
Amiodarone and Metabolite Serum 3ml serum from plain red top tube. Do not collect in SST. FREEZE. Collect no sooner than 12 hrs. after last dose. Therapeutic Range: 1.5-2.5 mg/l Chromatography (HPLC)
Amitriptyline and Nortriptyline (Elavil and Travail) 2ml serum from plain red top tube only. Do not collect in SST. Collect no sooner than 12 hrs after last dose. Therapeutic Range: 125-150mcg/l Chromatography (HPLC)
Ammonia

Plasma
3ml heparinized (green top tube) plasma. Send immediately on ice or FREEZE. 11-35 mmol/l (No normal range) Enzymatic Timed endpoint
Amoebiasis

Purged Stool
Submit stool sample in LV-PVA Fixative container. No ova or parasites found Microscopy
Amphetamine

Serum
5ml serum. Collect in plain red top tube. Negative GC/MS
Amphetamine

Urine
10ml random urine. Negative EIA
Amylase Isoenzymes

Serum ( includes total amylase
2mL serum. FREEZE serum in plastic vial. See lab report Enzymatic- Column Chromatography
Amylase

Serum

Urine

Fluid


1ml serum (SST)

10ml Aliquot of refrigerated 24-hour urine. Record total volume on requisition
3ml fluid


44-128 U/L

24-408 U/24 hrs.

Variable


Enzymatic

Enzymatic

Enzymatic
Androstenedione

Serum
2ml serum. Collect in plain red top tube. FREEZE serum in plastic vial.
See Addendum.
EXTRACTION, RIA
Angiotensin-1- Converting Enzyme

Serum
1ml serum.
Collect in plain red top tube.
8-52 U/L Spectrophometry (Kinetic)
Anti-A, Anti-B Titers See ABO Antibody Titer.    
Anti-Adrenal Cortex Antibodies

Serum
2ml serum. Refrigerate. None detected. IFA
Antibiotic Sensitivity Performed automatically on all relevant findings. N/A Disc Method a/o Breakpoint
Antibody Identification and Titer 1 pink top tube.
Do not use SST tube. Label test tube with patient’s full name.
N/A Gel technology
Antibody Screen Red Cell

(does not include identification and titer.) Phenotyping
1 pink top tube.
Do not use SST tube. Label tube with patient’s full name.
  Gel technology
Antibody Screen with Reflex to identification and Titer.
(No specimen will be saved for comparative titers.)
1 pink top tube.
Do not use SST tube. Label test tube with patient’s full name.
Negative Gel technology
Antibody Screen

(Pregnancy Monitoring)
1 pink top tube.
Do not use SST tube..Physician must provide mother’s date of birth.* Label test tube with patient’s full name.
Negative Gel technology
Anti-Cardiolipin Antibodies, lgA,lgG and lgM

serum
2ml serum.
Collect in plain red top tube.
< 10 SDU EIA
Anti-Convulsant Levels See individual drug listings    
Anti-Diuretic Hormone (Vasopressin)

Plasma
4ml Frozen EDTA plasma (lavender top tube). Draw into pre-chilled lavender top tubes. Separate plasma immediately into plastic vial and freeze. 1.0 – 13.3 pg/ml Extraction, RIA
Anti-DNA ( ds ) Antibodies

Serum
2 ml serum. <30 U/ml EIA
Anti-ENA (SSA/SSB, SM & RNP) Panel

Serum
2ml serum.
Collect in plain red top tube.
Negative EIA
Anti-Glomerular Basement membrane Antibodies (lgG)

Serum
2ml serum.
Collect in plain red top tube.
<5 units Immunoassay
Anti-Islet Cell Antibody

Serum
2ml serum.
Collect in plain red top tube.
None detected IFA
Anti-Mitochondrial Antibodies

Serum
2ml serum. <1:10 IFA
Anti-Myocardial Antibodies

Serum
2ml serum.
Collect in plain red top tube.
None detected IFA
Anti-Nuclear Antibody

Serum
1 ml serum. Negative EIA
Anti-Parietal Cell Antibodies

Serum
2 ml serum. <1:20 IFA
Anti-Platelet Antibody
Serum
2 ml serum.
Collect in plain red top tube.
None detected Flow Cytometry
Anti-Reticulin Antibodies

Serum
2 ml serum.
Collect in plain red top tube.
Negative IFA
Anti-Smooth Muscle Antibodies

Serum
2 ml serum. <1:20 IFA
Anti-Streptolysin 0 Titer 2 ml serum. <200 IU/ml Nephelometry
Anti-Striated (Skeletal) Muscle Antibodies

serum
2 ml serum.
Collect in plain red top tube.
None detected IFA
Anti-Thrombin III Activity One blue top tube.
Separate plasma from cells immediately. Transfer plasma to plastic vial and FREEZE.
78-115% of normal Chromogenic
Anti-Thyroid Antibodies

(Thyroglobulin and Peroxidase)
2 ml serum. Thyroglobulin Ab: <1.0 U/ml
Peroxidase Ab: <0.3 U/ml
Chemiluminescence
Arsenic

Blood


Urine


1 royal blue top tube (kit-493). Order KIT 493 from the lab.


7 ml aliquot of well mixed 24 hr urine. Add 20 ml 6N HCI to collection container prior to start of collection. Record total volume.


< 3 mcg/dl


<80 mcg/l


Mass Spectrometry


Mass Spectrometry
Ascorbic Acid See Vitamin C    
ASO See Anti-Streptolysin 0 Titer.    
Aspartate Amino Transferase (AST, SGOT)

Serum
1 ml serum (SST) 10-42 U/L Enzymatic
Aspergillus Antibodies

Serum

Screen

Titer
1 ml serum.
Collect in plain red top tube. Paired sera advisable to detect rising titers.




None Detected

<1:8
Immunodiffusion
Australian Antigen See Hepatitis B Surface Antigen    
Babesia Screen 1 lavender top tube. None seen. Microscopic Examination
Bacterial Antigens

H. Influenza, type b
N. Meningitidis group ACY w135
N. Meningitidis group B
S. Agalactiae (group B)
S. Pneumoniae
E. Coli K1
2 ml CSF, 2 ml serum or 10 ml random urine in sterile vial. Negative Latex Agglutination
Barbiturates

Blood
3 ml serum.
Collect in plain red top tube.

See Addendum
GC and FPIA
Barr Bodies

Buccal Smear
Smear of Buccal Mucosa fixed in 95% alcohol. N/A See page 4 for further instructions.
Bence Jones Protein See Immunoelectrophoresis, Urine    
Benzodiazepines

Serum

Urine


3 ml serum. Collect in plain red top tube.

25 ml random urine


Negative

Negative


HPLC

GC/MS
Beta-2 Microglobulin

Serum
1 ml serum <3.0 mg/l Nephelometry
Beta-HCG See Chorionic Gonadotropin (HCG).    
Bilirubin

Serum, Total

Serum, Direct

Serum, Indirect


1 ml serum. (SST)

1 ml serum. (SST)

1 ml serum. (SST)
0D 0-5.8 mg/dl 1D 0-8.2 mg/dl 5D 0.2-1.0 mg/dl

0.1-0.3 mg/dl

0.0-0.9 mg/dl


Colorimetry

Colorimetry

Calculated
Blastomyces Antibodies

Serum (Screen)
2 ml serum.
Collect in plain red top tube.
None detected Immunodiffusion
Bleeding Time, Ivy Please call Coagulation lab for appt. (670-1085) and instructions. 1-7 min. Functional
Blood Group and Rh Type See ABO Group and Rh Type.    
Blood Urea Nitrogen See Urea Nitrogen    
Brain Natriuretic Peptide
B-Type Natriuretic Peptide
5 ml frozen plasma from pre-chilled lavender top tube. Patient should be recumbent for 20 minutes prior to drawing blood. Place tube in ice and transport to lab.
See Addendum
RIA
Bromide

Serum
3 ml serum.
See Addendum.
Spectrophotometry
Brucella Agglutination

Serum
1 ml serum.

Paired sera advisable to detect rising titers.
<1:80 Agglutination
C-1 Esterase Inhibitor

Serum
1 ml serum.

Collect in plain red top tube. Separate and FREEZE immediately in plastic vial.
>68 % Nephelometry
C1Q Binding

Serum
2 ml serum.
Collect in plain red top tube. Separate serum and FREEZE in plastic vial.
0-13% Normal
13-16% Borderline
>16% Elevated
Radial Immunodiffusion
CA125 1 ml serum. 0.6 – 35.0 U/ml Chemiluminescence
CA15-3 - Sendout 1 ml serum.
Collect in plain red top tube.
< 32 U/ml. Chemiluminescence
CA19-9 - Sendout 2 ml serum.
Collect in plain red top tube.
< 37 U/ml Chemiluminescence
Calcitonin

Serum
1 ml frozen serum.
Collect in plain red top tube. Separate serum and FREEZE in plastic vial.
Male: <8 pg/ml
Female: <4 pg/ml
Chemiluminescence
Calcium

Serum, Total

Serum, Ionized

Urine


2 ml serum. (SST)

1 SST tube. Do not open.

50 ml aliquot of well mixed 24 hr. urine. Add 10 ml 6N HCI at beginning of 24 hr collection. Note 24 hr. volume on requisition.


8.5-10.5 mg/dl

1.12 - 1.320 mmol/L

100-300 mg/24° No range for random


Ion Specific Electrode

Ion Specific Electrode

Ion Specific Electrode
Calculus Analysis Submit specimen in a clean, sealed container. See report. Infrared Spectroscopy and X-Ray
Candida precipitins 10 ml serum.
Collect in plain red top tube.
None detected Immunodiffusion
Carbamazepine 2 ml serum.
Do not collect in SST.
Therapeutic Range: 8-12 mcg/mL. FPIA
Carbon Dioxide (CO2)

Serum
2 ml serum (SST) 24-31 mmol/l Ion Selective Electrode
Carbon Monoxide
(Carboxyhemoglobin)

blood
ABG Syringe
0-2% Co-Oximetry
Carcinoembryonic Antigen (CEA) 2 ml serum. 0 –5 ng/ml ECLIA
Sandwich Principle
Cardiolipin Antibody (IGA) 2 ml serum.
Collect in plain red top tube.
Negative <12 APL
Equivocal 12-15 APL
Elevated >15 APL
ELISA
Cardiolipin Antibody (IGG) 2 ml serum.
Collect in plain red top tube.
Negative <12 APL
Equivocal 12-15 APL
Elevated >15 APL
EIA
Cardiolipin Antibody (IGM) 2 ml serum.
Collect in plain red top tube.
Negative <12 APL
Equivocal 12-15 APL
Elevated >15 APL
EIA
Cardiovascular Evaluation See test Profiles    
Carotene

Serum
2 ml serum.
Collect in plain red top tube. Wrap in aluminum foil to protect from light.
50-300 mcg/dl HPLC
Catecholamines Fractionated, Serum

- Norepinephrine
- Epinephrine
- Dopamine
- Total catecholamines
2 pre-chilled green top tubes or 5 ml heparinized plasma. FREEZE. (Submit specimen on ice).
See Addendum
HPLC
Catecholamines, Fractionated, Urine

- Norepinephrine
- Epinephrine
- Dopamine
25 ml aliquot of well-mixed 24-hour urine. Separate serum and FREEZE in plastic vial. Add 25 ml 6N HCL to container at start of collection. Record total volume on both specimen container and request form.
See Addendum
HPLC
Cell Count Fluid 2 ml fluid.
Submit fluid in lavender top tube.
N/A Chamber Counts Cytoprep
Celontin See Methsuximide    
Ceruloplasmin

Serum
1 ml serum. 18-55 mg/dl. Nephelometry
Chlamydia Swab in M4 Transport Media or urine Negative PCR
Chloride

Cerebrospinal fluid

Serum

Urine


1 ml CSF.

1 ml serum. (SST)

50 ml aliquot of well mixed 24 hr. urine. Note 24 hr. volume on requisition.


122-132mmol/l

95-108mmol/l

110-250mmol/24 hr.


Ion Selective Electrode

Ion Selective Electrode

Ion Selective Electrode
Chlorpromazine

serum
See Thorazine    
Cholesterol

Total, Serum

High Density Lipoprotein (HDL)


1 ml serum. (SST)

3 m serum. (SST) Fasting specimen required.


120 – 200 mg/dl

(Chart)


Timed endpoint. Enzymatic

Direct Measurement
Cholesterol

Low Density Lipoprotein (LDL)


3 ml serum. Fasting specimen required.

See Addendum.
Calculation
Cholinesterase

Plasma
See Pseudocholinesterase    
Cholylglycine (Bile Acid)

Serum
3 ml frozen serum. Collect in plain red top tube. Transfer serum to plastic vial and FREEZE. Fasting specimen required. Fasting: <68 mcg/dl

Postprandial: Approx. 3x fasting
RIA
Chorionic Gonadotropin

Urine, Qualitative Pregnancy

Serum, Qualitative Pregnancy

Serum, Quantitative Pregnancy


10 ml first morning urine.

2 ml serum.

2 ml serum.


Negative

Negative

0-4.9 mIU/ml


Rapid Chromatographic Immunoassay

Rapid Chromatographic Immunoassay

ECLIA – Sandwich Principle
Chromium

Serum
2ml serum- dark blue top tube.
Obtain KIT 492 from lab. Transfer serum within 2 hours to aluminum free plastic vial (included in KIT 492).
£ 2.0 ng/dl Atomic Absorption
Citric Acid

Urine
10 ml aliquot from a well mixed 24 hr urine collection. Add 10 gm Boric Acid to container at start of collection. Female: 125-900 mg/24 hr.
Male: 65-650 mg/24 hr.
Spectrophotometry
Clonazepam 3 ml frozen serum.
Collect in plain red top tube. Transfer serum to plastic vial and FREEZE.
Therapeutic Range: 30-60 ng/ml

Partially Toxic: >100 ng/ml
HPLC
Clostridium Difficile Toxin A/B Assay

Stool
Submit stool specimen in sterile container. Specimens accepted Mon-Fri only. Negative ELISA
CMV See Cytomegalovirus titer.    
Coccidioidomycosis

Serum(Screen)
2 ml serum.
Collect in plain red top tube.
None detected Immunodiffusion
Cold Hemagglutinins 2 ml serum. Clot at 37ºC and separate serum from cells. DO NOT REFRIGERATE. <1:40 Agglutination
Complement C1Q Component

Serum
2 ml frozen serum.
Collect in plain red top tube. Transfer to plastic vial and FREEZE.
14-20 mg/dl. Radial Immunodiffusion
Complement C2

Serum
2 ml frozen serum.
Collect in plain red top tube. Transfer to plastic vial and FREEZE.
1.6-3.6 mg/dl Radial Immunodiffusion
Complement C3

Serum
1 ml frozen serum.
Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE.
83-177 mg/dl Nephelometry
Complement C4

Serum
2ml frozen serum.
Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE.
15-45 mg/dl Nephelometry
Complement C5

Serum
2 ml serum.
Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE.
7.3-19.0 mg/dl Radial Immunodiffusion
Female: 9-153 ng/ml
Complement C6

Serum
2 ml frozen serum.
Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE.
3.6-7.5 mg/dl Hemolytic Assay
Complement Specific Immune Complex

Serum
2 ml frozen serum.
Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE.
Normal: <15 mEq/ml
Borderline: 16-19 mEq/ml
Elevated: >20 mEq/ml
EIA
Complete Blood Count

With Differential

Without differential


1 lavender top tube.

1 lavender top tube.


See Addendum.

See Addendum.


Particle counter, Flow Cytometry and Microscopy

Particle counter.
Coombs, Direct

Blood
1 lavender top tube. Negative Immune Agglutination
Copper

Serum


Urine


2 ml serum. Collect using Kit-#492. Order KIT-492 from lab.


30 ml aliquot of well-mixed 24-hour urine. Indicate 24 hr. volume on requisition. MUST use special acid washed container and transfer vial (available through the lab).


70-155 mcg/dl


15-50 mcg/24 hr.


Atomic Absorption


Atomic Absorption
Cortisol

Free, Urine



Serum Cortisol


75 ml aliquot of well mixed 24 hr. urine. Add boric acid tablets at start of collection Note 24 hr. volume on requisition. FREEZE.



1 ml serum.


20 – 90mcg/24 hr.



2.3 - 19.4 ng/dl mg/dl


RIA



Chemiluminescence
Coxsackie A Virus Antibodies (2,7,10,16) 2 ml serum.
Collect in plain red top tube. Paired sera advisable to detect rising titers.
No antibody detected Complement Fixation
Coxsackie Virus Antibody (B1-B6) 2 ml serum.
Collect in plain red top tube. Fasting. Paired sera advisable to detect rising titers.
<1:8 Complement Fixation
C-Peptide

Serum
2 ml serum.
Collect in plain red top tube. Separate serum and FREEZE in plastic vial. Fasting sample required.
0.8-3.1 ng/ml Immunochemiluminometric
C-Reactive Protein (CRP)

Serum
2 ml serum. Negative Nephelometry
Creatine Kinase
Serum, Total (CK)

CK-MB Includes Total CK

2 ml serum (SST)

2 ml serum. FREEZE in plastic vial if not deliverable within 24 hours.

22-269 U/L

0-7 mcg/l

Enzymatic

Chemiluminescence
Creatinine Clearance 2 ml serum and 10 ml Aliquot of well-mixed 24-hour urine specimen. Note 24 hr. volume on requisition. 60-150 ml /min Jaffe Rate
Creatinine
Serum

Urine

2 m. serum. (SST)

25 mL aliquot of well mixed 24 hr. urine. Note 24 hr. volume on requisition.

0.5-1.5 mg/dl

1-2 gm/ 24 hrs

Jaffe Rate

Jaffe Rate
Cryoglobulins

Serum, Qualitative
2 ml serum.
Allow blood to clot at 37?C and separate serum. DO NOT REFRIGERATE, Transport at room temperature.
Negative Cold Precipitation
Cryptococcus Antigen

Serum

CSF


1 ml serum.

1 ml CSF.


Negative

Negative


Latex agglutination

Latex agglutination
Culture

T. Vaginalis
10 ml random urine No T. vaginalis recovered  
Culture

Throat for Beta-Strep only
Swab in transport media. Negative for beta strep  
Culture

Urine
Full sterile culture tube or sterile jar. No growth.  
Culture

Viral Isolation
Virus isolation transport media No growth  
Culture

Wound
Swab in transport media No growth  
Cultures

Acid Fast CSF


1 ml CSF in sterile tube.


No AFB recovered.
 
Cultures

Acid Fast Gastric


Gastric sample in sterile container.
No AFB recovered.  
Cultures

Acid Fast Misc.
Submit sample in sterile container No AFB recovered  
Cultures

Acid Fast Sputum
Sputum in sterile container. No AFB recovered  
Cultures

Acid Fast Urine
40 ml urine in sterile container. No AFB receovered  
Cultures

Anaerobes


Swab in Anaerobic Transport medium.


No growth
 
Cultures

B. Pertussis


Submit naso-pharyngeal culture on 2 Calgiswabs or cough plate


No B. Pertussis isolated
 
Cultures

Blood


5 ml blood in each of two-blood culture set bottles. Indicate diagnosis, antibiotic therapy, date and hour drawn on requisition.


No growth
 
Cultures

Body Fluid


Submit fluid in sterile container.


No growth.
 
Cultures

Cerebrospinal Fluid
Submit CSF in sterile container. No growth  
Cultures

Chlamydia
Submit swab in M4 Medium or first morning urine. Negative  
Cultures

Cryptosporidium
Stool Negative  
Cultures

Fungi
Submit material in sterile container or swab in transport media No growth  
Cultures

Genital
Swab in transport media. No growth  
Cultures

Gonorrhea
Swab in Amies charcoal media. No N. gonorrhea recovered.  
Cultures

Gonorrhea PCR
M4 or urine for male only Endocervical Negative  
Cultures

Herpes Simplex
Swab in viral transport media (M4). No growth.  
Cultures

Misc.
Swab in transport media. No growth  
Cultures

Mycoplasma
Swab in Mycoplasma transport media No mycoplasma recovered.  
Cultures

Nose/Respiratory
Swab in transport media Normal mouth bacteria  
Cultures

Sputum
Sputum in "Sealtight" container. Normal mouth bacteria  
Cultures

Stool
Stool in Cary and Blair media (yellow top container) No enteric pathogens isolated  
Cultures

Throat
Swab in transport media. Negative for beta strep  
Cultures


Acid Fast Blood
Note: All culture samples must be submitted according to instructions on page5.
10 ml blood in Dupont Isolator.



No AFB recovered.
 
Cyanide

Whole blood
2 gray top tubes. <0.1 mg/dl Gas Diffusion, Colorimetric
Cyclic Amp

Plasma




Urine


3 ml of EDTA plasma (lavender top tube). Separate plasma within 15 minutes of collection. Transfer plasma to plastic vial and FREEZE.




10 ml random urine. Acidify aliquot with 2 ml 6N HCL to keep pH below 3. FREEZE.


3.9 – 13.1 nmol/l




1.0 – 11.5 nmol/l


RIA




RIA
Cystine

Urine, Quantitative
25 ml aliquot of well-mixed 24-hour urine. Refrigerate while collecting. Acidify aliquot with 6N HCL to pH 2-3. Note 24-hour volume on requisition. Ship frozen. 10-100 mg/24 hr. HPLC
Cytgology

Smears
Slides fixed in 95% alcohol. N/A  
Cytology ThinPrep Specimen submitted in ThinPrep vial N/A Positive ASCUS reflexed to HPV  
Cytology

Buccal Smear
Smear of buccal mucosa fixed in 95% alcohol. N/A  
Cytology

Endometrial Wash
Specimen in equal volume of 50% alcohol. N/A  
Cytology

Gynecological (Includes Hormonal Evaluation)
Smear. DO NOT ALLOW SMEAR TO DRY. Spray smear with fixative or place slide into 95% alcohol. N/A  
Cytology

Rffusion, CSF
Specimen in equal volume of 50% alcohol. N/A  
Cytology

Sputum
Specimen in 50% alcohol. N/A  
Cytology

Urine
Submit specimen in equal volume of 50% alcohol. N/A  
Cytology


Aspirate fluid

Brushings
Note: Cytology specimens must be submitted according to instructions on Page 4.


Specimen in equal volume of 50% alcohol

Smear. DO NOT ALLOW SMEAR TO DRY. Spray smear with fixative or place slide into 95% alcohol.



N/A

N/A
 
Cytomegalovirus AB IgM 1 ml serum Negative ELISA
D-Dimer Assay

Plasma
D-Dimer Assay Plasma 2 ml Plasma.
Collect in blue top tube.
Cut off value <209 ng/ml Turbidimetric Immunoassay
Dehydroepiandrosterone (DHEA)

Unconjugated
1 ml serum.
Collect in plain red top tube.
Female: 130-980
Male: 180-1250 ng/dl
RIA
Dehydro-epiandrosterone Sulfate (DHEA-S) 1 ml serum.
See Addendum
Chemiluminescence
Delta Virus Antibody

IGG
IGM
2 ml serum Negative
Negative
EIA
Desipramine

Serum
2ml serum.
Collect in plain red top tube. Do not collect in SST.
Therapeutic Range: 50-300 mcg/l
Potentially toxic: >500 mcg/l
HPLC
Diazepam (Valium)

Serum
3 ml serum.
Collect in plain red top tube. Do not collect in SST.
Therapeutic Range: 0.5-2.0 mg/l
Potentially toxic: >3.0 mg/l
HPLC
Dibucaine Inhibition See Pseudocholinesterase    
Digitoxin (Digitalis)

Serum
1 ml serum.
Collect in plain red top tube. Do not collect in SST.
Therapeutic Range: 10-30 mcg/l
Potentially toxic: >30 mcg/l
RIA
Digoxin

Serum
2 ml serum.
Do not collect in SST
Therapeutic Range: 0.9-2.0 ng/ml Inverse Immunoturbidity
Dilantin See Phenytoin    
Direct Coombs
Blood
See Coombs, Direct, Blood.    
Doriden (Glutathimide)

Serum
3 ml serum.
Collect in plain red top tube. Do not collect in SST tube.
Therapeutic Range: 3.0-7.0 mg/l GC
Drug Screen, Comprehensive

Blood

Gastric

Urine


5 ml serum or 1 green top tube. Do not use SST.

10 ml gastric contents.

50 ml random urine.


None detected

Negative

Negative


Gas chromatography

Chromatography

MEIA
D-Xylose See Xylose Absorption.    
Echinococcus Antibody 2 ml serum.
Fasting specimen required. Paired sera advisable.
Negative ELISA
Elavil See Amitriptyline    
Electrolyte Panel See Test Profiles.    
Electrophoresis See individual listings    
Enterobius Vermicularis (Pinworm) Pinworm Paddle or scotch tape Negative Microscopic Examination
Eosinophil Count

Nasal Smear

Blood


Send air-dried smear on slide labeled with patient’s name.

1 lavender top tube.


0%

50-400 cells/cmm


Cytochemistry

Microscopic Examination
Epstein-Barr Virus Ab. Screen (Capsid IgG and IgM, Nuclear Antigen IgG) 2 ml serum. Specify age of patient on request form. See lab report. ELISA
Epstein-Barr Virus, DNA by PCR 2 ml EDTA plasma from lavender top tube < 200 copies/ml PCR
Erythrocyte Sedimentation Rate See Sedimentation Rate, Erythrocyte.   Westergreen Excyte (Automated)
Erythropoietin

Serum
2 ml serum. >16 years 4.0 –19.5 mU/ml Chemiluminescence
Estradiol

Serum
2 ml serum.
See Addendum
Chemiluminescence
Estriol

Serum
1 ml serum.
See Addendum.
RIA
Estrogen Fractionated

Includes Estrone and Estradiol Serum

Urine
2 ml FROZEN serum.

100 ml aliquot of 24-hour urine. Add 10 grams boric acid at start of collection. Record total volume on requisition.


See Addendum.
see lab report.


RIA

RIA
Estrone

Serum
4.0 ml frozen serum. Freeze in plastic vial.
Indicate patient’s age and sex on requisition.

See Addendum.
RIA
Ethanol See Alcohol, Ethyl    
Ethosuximide (Zarontin) 3 ml serum.
Collect in plain red top tube. Do not collect in SST.
Therapeutic Range: 40-100 mg/l RIA
Factor II Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
CSEPT - OUT
Factor IX Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor V Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor VII Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor VIII Antigen One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Electroimmunodiffusion
Factor VIII Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor X Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor XI Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor XII Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. 50-150% Photo-optical Clot Detection
Factor XIII Assay One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. Present Solubility
Fat, Stool See Lipids, Stool    
Ferritin

Serum
2 ml serum Female: 10-120 ng/ml
Male: 20-250 ng/ml
ECLIA – Electro Immunoassay Sandwich Principle
Fetal Erythrocytes See Kleihauer-Betke, Under Hemoglobin, Fetal.    
Fetal Hemoglobin See Hemoglobin, Fetal.    
Fibrinogen

Plasma, Quantitative
1 blue top tube.
Gently mix by inversion.
200 - 400 Photo-optical Clot Detection
Folic Acid

Serum
2 ml serum.
Fasting specimen preferred.
4.2-19.9 ng/ml
Age Dependent
ECLIA Electro Chemiluminescence Competition Principle
Follicle stimulating Hormone (FSH)

Serum
3 ml serum.
See Addendum.
MEIA
Francisella Tularensis Agglutination

Serum
2 ml serum. <1:80 Agglutination
Free Erythrocyte Protoporphyrins (FEP) 1 lavender top tube.
Gently mix by inversion.
<35 mcg/dl Fluorometry
Free Fatty Acids

Serum
2 ml EDTA plasma (Lavender top tube). Separate plasma and FREEZE in plastic vial. Adult 0.07-0.88 mmol/l Enzymatic Spectrophotometer
Free Testosterone See Testosterone, Free.    
Free Thyroxine

(Free T-4)
3 ml serum. 0.73-1.70 ng/dl. ECLIA – Competition Principle
Free Thyroxine

Index (T7)
See Thyroxine Index, Free    
G6PD, RBC 1 full lavender top tube 4.6 – 13.5 U/g HGB Enzymatic
Gamma Glutamyl Transferase (GGT) 1 ml serum. (SST) 0-130 U/L Enzymatic
Gastrin

Serum
2 ml serum.
FREEZE in plastic vial. Fasting for 12 hours is required.
<100 pg/ml RIA
Gentamicin

Serum
2 ml serum.
Do not collect in SST.
Trough 0-10 un/ml
Peak 10-12 un/ml
Particle Enhanced Turbidimetric Inhibition Immunoassay
Glucose Tolerance 3 hour Includes: Fasting, _, 1, 2 and 3 hours. Blood and Urine 5 gray top tubes. Indicate time on each tube and 5 urine samples. Gently mix tubes by inversion. N/A Oxygen Electrode
Glucose Tolerance, 6 hour Includes: Fasting, _, 1, 2, 3, 4, 5 and 6 hours. Blood and Urine 8 gray top tubes. Indicate time on each tube and 8 urine samples. Gently mix tubes by inversion. N/A Oxygen Electrode
Glucose

Blood, 2 hour Post Prandial

Blood, Fasting Plasma

Plasma, serum

Fluid

CSF, Quantitative

Urine, Quantitative


1 gray top tube. Indicate 2 hr post Prandial. Gently mix.

1 gray top tube. Gently mix.

3 ml serum. (SST)

Submit fluid in gray top tube.

1 ml CSF.

25 ml Aliquot of well-mixed 24-hour urine. Add 5 ml Glacial acetic acid at beginning of 24 hr. collection. Note 24 hr. volume on requisition.


70-140 mg/dl

70-115 mg/dl

70-115 mg/dl

Variable

40-80 mg/dl

0-0.3 g/24hrs (No range for random)


Oxygen Electrode

Oxygen Electrode

Oxygen Electrode

Oxygen Electrode

Oxygen Electrode

Oxygen Electrode
Glycohemoglobin (Hemoglobin A1c) 1 lavender top tube. 5.5-9.0 % A1c Liquid Chromatography HPLC
Gonadotropin, Chorionic See Chorionic Gonadotropin.    
Gram Stain for Gonococcus

(Males only)
Submit smear. Label with Patient’s name. Negative for GC Gram stain
Gram Stain Smear Submit smear. Label with Patient’s name. No organisms seen Gram stain
Growth Hormone 2 ml serum. Adult: <8 ng/ml
Child: <40 ng/ml
Chemiluminescence
Guaiac, Stool See Occult Blood, Stool.    
Haptoglobin 2 ml serum. 50-170 mg/dl. Nephelometry
HCG See Chorionic Gonadotropin.    
HDL Cholesterol See Cholesterol HDL.    
Heavy Metal Screen, Urine. Includes:
Arsenic
Lead
Mercury
90 ml aliquot of well mixed 24 hour urine collection (Acidify to pH of 2 with 20 ml 6N HCL) Record total volume on requisition. Arsenic: <80 mcg/dl
Lead: <50 mcg/dl
Mercury: up to 20 mcg/dl
Atomic Absorption
Atomic Absorption
Atomic Absorption
Helicobacter Pylori Ab. One red top tube Negative EIA
Hemoglobin A1c See Glycohemoglobin.    
Hemoglobin S, Screen See Sickle Cell.    
Hemoglobin Variant 1 lavender top tube.
Invert several times.

See Addendum
Ion exchange HPLC
Hemoglobin, Fetal

Kleihauer-Betke Qualitative

Quantitative (Fetal Erythrocytes)


1 lavender top tube. Invert several times.

1 lavender top tube. Invert several times.


0-3%

0-3% Fetal


Acid elution-Smears

Acid elution-Smears
Hemoglobin, Unstable 1 lavender top tube. Negative Isopropanol Solubility
Hemosiderin
25 ml aliquot of first morning urine specimen. Negative Potassium Ferrocyanide-HCL
Urine, Qualitative 10 ml Urine Specimen. No Preservative   Clinitek Atlas Automated Reflectance Spectrophotometer
Hepatitis A (HAV)

Antibody, Total

IgM Antibody


2 ml serum

2 ml serum


Negative

Negative


EIA

EIA
Hepatitis B (HBV)

Core Antibody, Total

Core IgM Antibody

DNA, Qualitative PCR


DNA, Quantitative PCR

e Antibody (HBeAb)

e Antigen (HBeAg)

Surface Antibody (HBsAb), Qualitative

Surface Antigen (HBsAg)


2 ml serum

2 ml serum

1ml frozen EDTA plasma from lavender top tube. Transfer to plastic vial. FREEZE.


1 ml serum. Collect in plain red top tube. Separate and FREEZE.

2 ml serum

2 ml serum

2 ml serum

2 ml serum


Negative

Negative

None Detected


None Detected

None Detected

Negative

Negative

Negative


EIA

EIA

PCR


PCR

EIA

EIA

EIA

EIA
Hepatitis C (HCV)

Antibody

Antibody by Immunoblot Assay (RIBA)


RNA by Branched DNA Assay

Hep. C RNA QT PCR/TMA

Hep. C Genotype


2 ml serum

1 ml serum. Must be free of hemolysis and lipemia.

1 ml FROZEN serum. Must separate and freeze within one hour after collection or 1ml frozen EDTA plasma.


4ml frozen plasma (draw 2 lavender top tubes) or 4ml frozen serum.

4ml frozen plasma (draw 2 lavender top tubes) or 4ml frozen serum.


Negative

Negative

<0.2mEq /ml


Negative


EIA

Immunoblot

Branched DNA Amplification


PCR/TMA

PCR, DNA Sequencing
Herpes Simplex (HSV)

Types 1 and 2, IgG Antibody
1 ml serum HSV1: £0.90
HSV2: £0.90
EIA
Herpes Simplex (HSV)

Types 1 and 2, IgM Antibody
1 ml serum HSV1: < 1/10
HSV2: < 1/10
IFA
Herpes Zoster See Varicella.    
Heterophile Screen 1 ml serum Negative Agglutination
Heterophile Titer Serum 2 ml serum. Negative Agglutination
Hexosaminidase Studies See Tay-Sachs    
High Density Lipoprotein See Cholesterol HDL    
Histamine

Blood
1 green top tube.
Transfer plasma to plastic vial and FREEZE.
9-141 ng/ml RIA
Histoplasma Antibodies

Serum
1 ml Serum
Paired sera advisable to direct rising titers.
Negative Complement Fixation
Histoplasma Antigen Urine Negative  
HIV(Human Immunodeficiency Virus) HIV 1/2

Antibody Screen with automatic confirmation of equlvocal and Positive results by Westen Blot
1 red top tube.
Must use HIV requisition. Contact laboratory for special forms.
Non-reactive Screen: EIA
Confirmation: Western Blot
HIV-1 Genotyping

HIV-1 RNA, PCR 1st Gen


HIV-1 RNA, PCR 2nd Gen.


HIV-1 RNA, BDNA 3rd Gen.
2 ml frozen EDTA plasma from Lavender Top tube.
Transfer plasma to plastic vial and FREEZE.
See report

<400 copies/ml
<2.6 log copies/ml

<50 copies/ml
<1.7 log copies/ml

< 75 copies/ml
< 1.9 log copies/ml
Reverse Transcriptase PCR and DNA sequencing.

Reverse transcription- DNA Amplification



Branched DNA Signal Amplification
HLA-B27 Antigen 2 full yellow (preferable) or two green top tubes (whole blood)
Maintain at room temperature. DO NOT REFRIGERATE. Submit Monday-Thursday only. Include patient’s DOB. Sex and possible diagnosis. Invert tubes several times.
Negative Flow Cytometry
Homovanillic Acid (HVA)

Urine, Quantitative (includes creatinine)
10 ml Aliquot from well mixed 24 hr. collection. Add 25 ml 6N HCL to container at start of collection. Fecord total volume on requisition. 0-10 mg/24 hr.
Adults:
1.6 – 7.5 mg/24 hr.
HPLC
HTLV I/II Antibody

Serum
2 ml serum Negative EIA
Human Growth Hormone See Growth Hormone    
Human Papillomavirus (HPV)

DNA Test
Cervical swab or Frozen Biopsy in ViraPap Transport media. Collect in digene kit Negative DNA Hybridization
Hydroxyproline

Urine, Free
(includes creatinine)




Urine, Total


50 ml aliquot of well-mixed 24-hour urine. Add 10 ml HCL to container at start of collection. Refrigerate. Note 24 hour volume on requisition.


50 ml aliquot of well-mixed 24-hour urine. Add 10 ml HCL to container at start of collection. Refrigerate. Note 24 hour volume on requisition.


< 1.6 ug/ml (for random)




Males: 9-73 mg/24 hr.
Female: 7-49 mg/24 hr.


Spectrophotometry




Spectrophotometry
Imipramine and Desipramine

Serum
3 ml serum. Do not collect in SST. Therapeutic Range:
150-300 mcg/l
HPLC
Immune Complex (Raji Cells) 1 ml serum.
Collect in plain red top tube. Separate and FREEZE immediately in plastic vial.
<24 ug/ml - Negative
>=24 ug/ml - Positive
EIA
Immunofixation

Serum



Urine


2 ml serum. Please provide age and diagnosis.

200 ml aliquot of well mixed 24 hour urine collection Note 24 hour volume on requisition. Please provide age and diagnosis.


Report includes electrophoretic pattern, interpretation, Total Protein, IgG, IgA, and IgM.



Negative


Nephelometry



IEF
Immunoglobulin A (IgA)

Serum. Quantitative
1 ml serum 70-312 mg/dl Nephelometry
Immunoglobulin D (IgD)

Serum. Quantitative
1 ml serum 0-14 mg/dl RID
Immunoglobulin E (IgE)

Serum. Quantitative
3 ml serum <120 IU/ml EIA
Immunoglobulin G (IgG)

Serum. Quantitative

CSF, Quantitative


1 ml serum

2 ml CSF. FREEZE in plastic vial


609-1349 mg/dl

0.5-6.1 mg/dl


Nephelometry

Nephelometry
Immunoglobulin G (IgG)

Subclasses
3 ml serum FREEZE in plastic vial. See report RID
Immunoglobulin M (IgM)

Serum. Quantitative
1 ml serum. 56-352 mg/dl Nephelometry
Infectious Mononucleosis See Heterophile Titer.    
Influenza A/B

Antigen
Nasopharynx swab in viral transport Negative FIA
Influenza A+B Nasopharyngeal Swab in viral transport Negative FIA
Influenza Antibody

(Types A&B) serum
2 ml serum. Paired sera advisable to detect rising titers. <1:8 Complement Fixation
Insulin Antibody

Serum
2 ml fasting serum. None Detected Radiometric
Insulin

Serum
2 ml fasting serum. FREEZE in plastic vial. <17.0 mIU/ml RIA
Iron-Total, Iron Binding Capacity And Saturation 4 ml serum. Avoid hemolysis. Total Iron:
0D=100 – 250 mg/dl
1D= 50 – 120 mg/dl
13D= 40 – 160 mg/dl
Male: 55-200 mg/dl
Female: 35-200 mg/dl
TIBC: 250-425 mg/dl
% Saturation: 20-55%
Spectrophotometry Timed endpoint
Ketones

Serum, Qualitative

Urine, Quantitative


2 ml serum

5 ml urine


Negative

Negative
Colorimetry
KOH Preparation Submit sample in sterile tube Negative KOH digestion
Lactic Acid (Lactate)

Plasma, Quantitative


3 ml plasma.
Draw blood in grey top tube. Collect on ice. Centrifuge, separate plasma


Venous: 0.5-2.2 mmol/l
Arterial: 0.5-1.6 mmol/l
Enzymatic
Lactic Dehydrogenase (LDH)

Serum

Fluid


2 ml serum. (SST)

1 ml fluid


91-180 U/L

Variable


Enzymatic

Enzymatic
Lactic Dehydrogenase (LDH)

Serum

Fluid


2 ml serum. (SST)

1 ml fluid


91-180 U/L

Variable


Enzymatic

Enzymatic
Lactic Dehydrogenase

Isoenzymes-1 Includes Total LDH
1 ml serum 25-70 U/L EIA
Lamotrigine 1 ml serum.
Collect in plain red top tube
4.0 – 18.0 ug/ml HPLC
LASA (Lipid Associated Sialic Acid) 2 ml serum.
Collect in plain red top tube.
109-262 mcg/dl Colorimetry
Latex Fixation See Rheumatoid Factor.    
LDL Cholesterol See Cholesterol LDL    
Lead

Blood




Urine


Whole blood-either 1 brown top tube or 1 lavender top tube. Mix gently by inversion.

25 ml aliquot of well-mixed 24-hour urine. Collect in lead free container containing 20 ml of 6N HCL. Note 24 hour volume on requisition. Random urine acceptable.


<25 mcg/dl >10 mcg/dl should be investigated in children under 6 yrs.

0-80 mcg/l


Atomic Absorption/ Graphite Furnace



Atomic Absorption
Lecithin/Sphingomyelin Ratio 10 ml amniotic fluid. FREEZE in plastic vial. See report TLC
Legionella (genus)

DFA
Submit specimen in sterile container. Plus DOH form Negative Fluorescent Stain
Legionella Antibody Pneumophila Antibody (Legionnaire’s Disease)

Serum
1 ml serum. Collect in plain red top tube. Paired sera advisable to detect rising titers. < 1:64 IFA
Legionella Antigen Urine in sterile container. None Detected EIA
Legionella Pneumophila (Legionnaire’s Disease)

Culture
Submit specimen in sterile container. Negative Culture
Leptospira Agglutination 2 ml serum.
Collect in plain red top tube.
Negative Indirect Hemagglutination
Leucine Aminopeptidase

Serum
1 mL serum.
Collect in plain red top tube. Do not use SST tube
8-22 mU/ml at 25?C Spectrophotometry
Light Chain

Urine
See Immunofixation    
Lipase Serum 2 ml serum 4-24 U/dl. Timed Enzymatic Rate
Lipids, Stool

Qualitative

Quantitative


Random stool specimen.

Secure special collection container and instructions from laboratory. SHIP FROZEN.


Negative

1-7 gm/24 hrs.


Sudan IV stain Microscopy

Gravimetric
Lithium

serum
1 ml serum. Collect in plain red top tube. Therapeutic range: 1.0 – 1.20 Spectrophotometers Endpoint Rate
Liver Function Panel See Test Profiles    
Low Density Lipoprotein(LDL) See Cholesterol LDL.    
Lupus Anticoagulant 1 blue top tube. Ratio interpretation 1.1or less not detected Photo optical Clot detection
Luteinizing Hormone (LH)

Serum
2 ml serum. State patient’s sex and age on request form.
See Addendum.
EIA
Lyme Disease Antibody 1 ml serum.   EIA
Lysozyme (Muramidase)

Serum
1 ml serum. (SST) 4.7-14.5 mcg/ml Radial Immunodifusion
Magnesium

Serum
1 ml serum. (SST) 0D= 1.5 – 1.9 mg/dl
13D= 1.7 – 2.5 mg/dl
Timed endpoint Colorimetry
Malaria, whole Blood 1 lavender top tube or thick and thin blood smears. No Malarial Parasites seen Microscopic Examination
Marijuana Screen

Urine
10 ml random urine. Negative EIA
Measles Antibody

Serum
1 ml serum. Paired sera advisable to detect rising titers. Negative EIA
Mebaral, (mephobarbital) 3 ml serum. Collect in plain red top tube. Do not use SST tube Therapeutic Range:
15-40 mg/l
Gas Chromatography
Mellaril, (Thioridazine and Mesoridazine) 5 ml serum. Collect in plain red top tube. Do not use SST tube Therapeutic Range:
0.2-2.6 mg/l
Gas Chromatography
Meprobamate, (Miltown) 3 ml serum. Collect in plain red top tube. Do not use SST tube Therapeutic Range:
5.0-30.0 mg/l
Gas Chromatography
Mercury

Blood








Urine


2 ml Heparinized Plasma. Dark blue top trace metal tube. Obtain KIT 493 from lab. Transfer plasma within 2 hours to aluminum-free plastic vial (included in KIT 493).



50 ml aliquot of well-mixed 24 hr. urine to special Acid washed transfer tube. Do not add preservative to 24 hr. urine container. Container must also be Acid washed. Contact CDL for special Acid washed container. Note 24 hr volume on requisition. Random urine also acceptable.


<10 mcg/dl







<20 mcg/l Acceptable range for industrial exposure: <150 mcg/l


Mass spectrometry








Mass spectrometry
Mesantoin (Mephenytoin) 2 ml serum. Collect in plain red top tube. Do not collect in SST Therapeutic Range:
15-40 mg/l
HPLC
Metanephrines, Fractionated

Urine
25 ml aliquot from a well-mixed, 24 hr. collection. Add 30 ml 6N HCL to container at the start of collection. Record total volume on both the specimen container and the test form

Total Metanephrines: 0.0 – 1.30 mg/24hrs
Metanephrine: 0.0 – 0.40 mg/24hrs
Normatenephrine:
0.0 – 0.90 mg/24hrs
HPLC
Metanephrines, Fractionated

Plasma
4 ml frozen EDTA plasma from Lavender Top tube. Transfer plasma to plastic vial and FREEZE.

Metanephrine: < 0.90 nmol/l
Normetanephrine: < 0.50 nmol/l
HPLC
Methemoglobin 1 Green top tube (Heparin).
Assay must be performed within 4 hours of venipuncture. Maintain sample on ice.
< 2.0% HGB Colorimetry
Methsuximide 2 ml serum. Collect in plain red top tube. Do not collect in SST Therapeutic Range:
10-40 mg/l
HPLC
Methylphenidate 1 ml serum. Collect in plain red top tube. Separate and FREEZE immediately in plastic vial. 5 –40 ng/ml HPLC
Mexiletene 2 ml serum. Collect in plain red top tube. Do not collect in SST Therapeutic Range:
0.7-2.0 mg/l
HPLC
Micro Hemagglutination Antibodies (MHA-ABS) 1 ml serum Non-reactive IFA
Microfilaria 1 lavender top tube. Negative Microscopic Exam
Microsporidia Stain Stool Negative Microscopic Exam
Mumps Virus Antibody

Serum
1 ml serum. Paired sera advisable to detect rising titers. Negative EIA
Mycoplasma Pneumoniae Antibody, Serum

IGG

IGM
2 ml serum. Paired sera advisable. 11 Negative

Negative
EIA
Mycoplasma Pneumoniae Isolation See Culture, Mycoplasma.    
Myoglobin

Serum

Urine, Qualitative


2 ml serum

20 ml Random urine


<60 mcg/l

Negative


Nephelometry

Nephelometry
Mysoline See Primidone    
Nortriptyline

Serum
2 ml serum. Collect in plain red top tube. Do not collect in SST Therapeutic Range: 50-140 mcg/l HPLC
N-Telopeptide (NTx")

Serum



Urine


2 ml serum. Collect in plain red top tube. Do not collect in SST

2 ml urine from 2nd morning void. Collect in sterile container.


Females: 14 – 76
Males: < 86


EIA

EIA
Occult Blood

Stool
Random stool specimen. Negative Guiac Paper Test
Osmolality

Serum

Urine


2 ml Serum.

10 ml Random urine.


280-305 mOsm/kg

40-1400 mOsm/kg (no range)


Freezing Point Depression

Freezing Point Depression
Ova and Parasites

Stool
10 gms. Random stool in 10% formalin. None seen. Microscopic Examination
Oxalate

Urine
25 ml aliquot of well-mixed 24-hour urine. Add 30 ml 6N HCL to container at start of collection. PH must be <3. Refrigerate. Record total volume on requisition. 0-40 mg/24 hrs. Spectrophotometry
Pap Smear See Cytology.    
Parainfluenze Antiboldy

(Types 1,2,3) Serum or CSF
1 ml serum or CSF Paired sera advisable to detect rising titers.

Serum: <8

CSF: <2
Complement Fixation
Parasite Identification Specimen in alcohol. (Not stool) N/A Microscopic Examination
Parathyroid Hormone(PTH) C-TERMINAL/ MIDREGION

Includes Total Calcium
5 ml serum. FREEZE. Please indicate if patient has renal disease. 50-340 pg/ml
Calcium: 8.5-10.6 mg/dl
RIA
Parathyroid Hormone-Intact

Serum
1ml serum. FREEZE in plastic vial. 2 ml EDTA plasma also acceptable 12 – 72 pg/ml
Calcium: 8.5-10.6 mg/dl
ECLIA Immunochemiluminometric Sandwich Principle
Partial Thromboplastin Time, Activated (aPTT) 1 blue top tube.
Tube must be properly filled to avoid dilution error. Mix thoroughly by gentle inversion.
21-34.4 seconds Photo-optical Clot Detection
Pathology,Surgical See Tissue Pathology    
Phenobarbital

Serum
2 ml serum. Do not collect in SST. Therapeutic Range:
15-40 mg/l
Particle enhanced turbidmetric Inhibition Immunoassay
Phenytoin 1 ml serum. Do not collect in SST. Therapeutic Range:
10-20 mg/l
Particle enhanced turbidmetric Inhibition immunoassay
PhosphatidylGlycerol1 ml amniotic fluid 1 ml amniotic fluid. Positive indicates fetal maturity Immunoagglutination
Phosphorus

Serum


Urine


1 ml serum.


25 ml aliquot of well mixed 24 hr. urine. Add 10 ml 6N HCL at beginning of 24 hr. collection . Record 24 hr volume on requisition.


Female: 2.6-4.4mg/dl
Male: 2.5-6.0mg/dl

0.3-1.3g/24 hrs.


Colorimetry – Timed endpoint


Colorimetry
Phosphtidylserine (IgG, IgM) 1ml plasma from a light blue top tube. gG- Negative

IgM- Negative
EIA
Pinworm See Enterobius Vermicularis    
Platelet Count

Quantitative
1 lavender top tube.
Mix by inversion.
150-450 k/uL. Particle Counter
Platelet Function Assays See Coagulation Studies    
Pneumocystis carinii Sputum in sterile container. Negative Immunofluorescence
Polio Virus Antibody

Types 1,2,3 Serum
3ml serum. Paired sera advisable to detect rising titers. <1:4 Neutralization
Porphobilinogen

Urine, Quantitative
15ml aliquot of well mixed 24 hr. urine. Refrigerate while collcting. FREEZE. Wrap container in aluminum foil to protect it from light. Note 24 hr. volume on requisition. < 2.7 mg/24hr. Column Chromatography and Spectrophotometry
Porphyrins

Feces, Quantitative
100 grams Feces from 48-72 hour collection. Send entire collection FROZEN.
See Addendum.
HPLC
Porphyrins

Urine, Quantitative Includes: Coproporphyrins and Uroporphyrins
15 ml aliquot of 24 hr. urine. Refrigerate during collection. Protect from light by wrapping with aluminum foil. Record 24 hr. volume on requisition.
See Addendum
HPLC
Potassium

Fluid

Serum

Urine


1 ml fluid (State source)

1 ml serum. (SST)

25 ml aliquot of well mixed 24 hr. urine. Record 24 hr. volume on requisition.


2.0-5.0 mmol/l

3.5-5.3 mmol/l

25 – 125 mmol/24 hr.


Ion Selective Electrode

Ion Selective Electrode

Ion Selective Electrode
Pregnancy Test

Serum
See Chorionic Gonadotropin, Urine, Qualitative    
Primidone

Serum
2ml serum. Collect in plain red top tube. Do not collect in SST. 5-12 mcg/l EIA
Procainamide (Pronestyl) and NAPA

Serum
5ml serum.
Collect in plain red top tube. Do not collect in SST.
4-10 mcg/l FPIA
Progesterone

Serum
4 ml serum.
See Addendum
RIA
Prolactin

Serum
3 ml serum. F = 3.4 – 24.4
M= 4.1 – 18.4
ECLIA Sandwich Principle
Prostate Specific Antigen

Serum (PSA)
1 ml serum. 0-2.5 ng/ml ECLIA Sandwich Principle
Protein C, Activity One full blue top tube. Separate plasma and FREEZE in plastic vial. 60-180% Chromogenic Detection. Photo Optical
Protein C, Antigen One full blue top tube. Separate plasma and FREEZE in plastic vial. 70-140 % of Normal EIA
Protein Electrophoresis
Cerebrospinal fluid

Serum

Urine

5 ml CSF

1 ml serum.

50 ml random urine


See Addendum

See Addendum
total protein: negative

Electrophoresis

Electrophoresis

Electrophoresis
Protein S, Activity One full blue top tube. Separate plasma and FREEZE in plastic vial. Male: 70-150%
Female: 60-140%
Chromogenic Photo Optical
Protein S, Antigen One full blue top tube. Separate plasma and FREEZE in plastic vial. 70-140 % of Normal EIA
Protein, Total

Fluid

Cerebrospinal Fluid

Serum

Urine


2 ml fluid. (State source.) 2ml CSF.

1 ml serum.

100 ml aliquot of well mixed 24 hr. urine. Record 24 hr. volume on requisition.


0-6 gm/dl (No range)

15-45 mg/dl

Female: 6.7-8.6 gm/dl
Male: 6.2-8.1gm/dl

Female= 50-100 mg/24hrs
Male= 20-150 mg/24hrs


Colorimetry biuret

Colorimetry

Colorimetry

Colorimetry
Prothrombin Time (PT) 1blue top tube. Tube must be properly filled to avoid dilution error. Mix gently by inversion 5 or 6 times. 11-13 seconds Photo-optical Clot Detection
Pseudocholinesterase 2 ml EDTA plasma.
(Lavender top tube).
3200-6600 U/L Spectrophotometry
PTH See Parathyroid Hormone.    
Pyruvate

Blood
5 ml protein free filtrate. Collect specimen in a gray top tube. Mix well. Immediately add 4 ml whole blood to 4 ml of 7% perchloric acid and mix. Let stand in the refrigerator for 5-10 minutes to ensure complete precipitation of protein. Centrifuge and submit clear supernatant in plastic vial. 0.3-0.9 mg/dl Spectrophotometry
Quinidine

Serum
2ml serum from plain red top tube only. Do not collect in SST. 2.3-5.0 mcg/ml FPIA
RAST See allergy testing.    
Renin

Plasma
One Blue top tube. Separate plasma and FREEZE in plastic vial.
See Addendum.
Chemiluminescence
Reptilase Time 1 Blue Top Tube <23 seconds Tilt Tube
Respiratory Syncytial Virus (RSV) Direct Antigen Nasopharygeal swab placed in M-4 transport medium. Indicate source on requisition form and on specimen container. Not Detected EIA
Reticulocyte Cell Count 1 lavender top tube.
Mix gently by inversion.
0.1-2.5% Microscopic Examination and Flow Cytometry
Rh Phenotype See ABO Group and Rh Type.    
Rheumatoid Factor

Serum
2 ml serum. < 20 IU EIA
Ristocetin One 5 ml blue top tube. Mix. Separate plasma and FREEZE in plastic vial. 42-200 %Normal Platelet Agg.
Rotavirus Stool in plain sterile container negative ELFA
Rubella Antibody

Screen

Titer


2 ml serum.

2 ml serum. Paired sera advisable to detect rising titers.
Immune: >15 IU
Equivocal: 10-15 IU
NOT Immune: <10 IU


EIA

EIA
Rubeola See Measles    
Saccharomyces Cerevisiae-IgG 3 ml serum. Do not collect in SST. Negative: £ 20 Units
Equivocal: 20.1- 29.9 Units
Positive: >= 30 Units
 
Salicylates

Serum
3 ml serum. Do not collect in SST. 4-100 mg/dl (No range) Timed endpoint
Sedimentation Rate, Erythrocyte 1 lavender top tube.
Mix by gentle inversion.
Female: 0-20 mm/hr.
Male: 0-15 mm/hr.
Westergreen
Excyte Automated
Semen Analysis

Complete
Collect entire specimen in clean jar or test tube. Must reach lab within 3 hours of collection. Note collection time and date on requisition. Maintain specimen at 37?C.
See Addendum.
Microscopic and Macroscopic Examination
Serotonin

Blood, Quantitative




Urine, Qualitative


One 5 mL. EDTA/Ascorbic Acid whole blood. FREEZE in plastic vial. (Speckled red/yellow top tube)

See 5 Hydroxyindoleacetic Acid
Adult: 50-175 ng/ml HPLC
Sex Chromatin See Barr Bodies, Buccal Smear.    
SGOT See Aspartate Amino Transferase   Enzymatic
SGPT See Alanine Amino Transferase.   Enzymatic
Sickle Cell Screen

Blood
1 lavender top tube.
Mix by gentle inversion.
Negative Solubility
Sirolimus (Rapamycin) 2 ml whole blood from a lavender top tube (EDTA). 3.0-18.0 ng/ml Liquid Chromatography/ Tandem Mass Spectrometry
Sjogren’s Antibody See Anti-ENA Panel   Immunoassay
Sodium

Fluid

Serum

Urine, 24 hr.


Urine, Random


2 ml fluid.

1ml serum. (SST)

20 ml aliquot of well mixed 24 hr. urine. Record total volume on requisition.
20 ml random urine collection.


Not Established

135-145 mmol/l

43-217mmol/24 hr.


Not Established


Ion Selective Electrode

Ion Selective Electrode

Ion Selective Electrode


Ion Selective Electrode
Somatomedin C
(Insulin-Like Growth Factor-I)
3 ml frozen serum.
Do not collect in SST. Red Top Tube Only


See Addendum.
Chemiluminescence
Specific Gravity

Fluid
1 ml fluid. (Specify source) Variable Refractometry
Sperm Count See Semen Analysis    
Stain, Esterases (Specific and non-specific) Blood smear or bone marrow smear. Label slide with patient’s name See individual Report. Histochemical stain, microscopy
Stain, Periodic Acid Schiff (PAS Blood smear or bone marrow smear. Label slide with patient’s name. See individual Report Histochemical stain, microscopy
Stain, Peroxidase Blood smear or bone marrow smear. Label slide with patient’s name. See individual Report. Histochemical stain, microscopy
Stain, Sudan Black B Blood smear or bone marrow smear. Label slide with patient’s name. See individual Report. Histochemical stain, microscopy
Stain, Tartrate Resistant Acid Phosphatase Blood smear or bone marrow smear. Label slide with patient’s name. See individual Report. Histochemical stain, microscopy
Stain, TdT Blood smear or bone marrow smear. Label slide with patient’s name. See individual Report. Immunofluorescence
Stone Analysis See Calculus Analysis.    
Streptozyme Screen 2 ml serum (SST) Negative Immunoassay
Synovial Fluid Analysis

Includes WBC count, differential crystal analysis, culture, smear, and uric acid
1 ml fluid in sterile tube, plus 5 ml fluid in red top tube and 2 ml fluid in lavender top tube. Mix by inversion
See Addendum.
Microscopic Examination
Syphilis Serology (RPR) 1 ml serum. Non-reactive Rapid Plasma Reagin Test
T and B Cell Enumeration 1 lavender top tube plus 1 yellow top tube. Ship at room temperature. Report includes Interpretation. Flow Cytometry
T3 See Triiodothyronine.    
T4 See Thyroxine.    
Tay-Sachs Carrier Screen, Non-pregnant
Serum
5 ml serum. Do not collect blood in SST tube. Place on ice immediately after drawing. Separate serum. FREEZE in plastic vial. See report. Enzymatic Fluorometry
Tay-Sachs Carrier Screen, Pregnant
Platelets
5 blue top tubes. Invert gently 5 times. Refrigerate. Do not centrifuge. See report. Enzymatic Fluorometry
Tegretol See Carbamazepine    
Testosterone

Free/Weakly Bound And Total
5 ml serum.
Specify patient’s age and sex.
Males
Total: 260-1000 ng/dl
Free: 50-210 pg/ml
Percent Free testosterone: 1.0-2.7

Females
Total: 15-70 ng/l.
Free: 1.0-8.5pg/ml
% Free Testosterone: 0.5-1.8%
RIA
Theophylline

Serum
1 ml serum.
Do not collect in SST.
Therapeutic Range:
10-20 mg/dl
Particle enhanced turbidimetric immunoassay
Thorazine (Chlorpromazine)

Serum
4 ml serum. Do not use SST. Protect from light by wrapping in aluminum foil. Therapeutic Range:
30-350mg/l
Gas Chromatography
Thrombin Time 1 blue top tube. Fill to avoid dilution error. Mix gently by inversion. 9.4-13.0 seconds. Photo-optical Clot Detection
Thyroglobulin 2 ml serum 0-42mg/ml Chemiluminescence
Thyroglobulin Ab. 2 ml serum Negative Chemiluminescence
Thyroid Stimulating Hormone 3 ml serum 0.27 – 4.2mIU/ml ECLIA Sandwich Principle
Thyroxin Free (Free T4)
Serum
2 ml serum 0.93 – 1.7ng/dl ECLIA Competition Principle
Thyroxine Binding Globulin (TBG) 1 ml serum 16-34 mg/ml RIA
Thyroxine, T4 2 ml serum 5.1-11.1mg/dl ECLIA Competition Principle
Tissue Pathology Submit specimen in buffered formalin solution. Submit Bone Marrow in Bouin’s Solution.    
Tissue Transglutaminase Ab. IgA 2 ml serum. Do not collect in SST. Negative: <20 Units
Weak Positive: 20-30 Units
Mod – Strong Pos: >30 Units
EIA
Tobramycin

Serum
2 ml serum. Do not collect in SST.

Trough: 1-2 mg/ml

Peak: (No range)
Particle enhanced turbidimetric inhibition immunoassay
Toxoplasma Antibodies 1 ml serum. <10 IU EIA
Transferrin

Serum
2 ml serum 215-380 mg/dl Spectrophotometry - endpoint
Treponemal Antibody Test
Syphilis
See Fluorescent Treponemal Antibodies FTA.    
Trichomonas Screen

Urine
10 ml random urine. Negative Microscopic Examination
Triglycerides

Serum
3 ml serum (SST) Ages 0D= 20 –150 mg/dl
29D= 46 – 200 mg/dl
39D= 46 – 236 mg/dl
Enzymatic – Timed endpoint
Triiodothyronine (T3)

Serum
3 ml serum. 0.8-2.0 ng/ml ECLIA – Competition Principle
Urea Nitrogen
Serum

Urine, 24hr





Urine, Random

1 ml serum. (SST)

10 ml aliquot of well-mixed 24-hour collection. Refrigerate during collection. Note 24-hour volume on requisition.

10 ml random urine

5-25 mg/dl

5.0-20.0g/24 hrs.





(No range for random urine)

Enzymatic-Electrode

Enzymatic-Electrode





Enzymatic-Electrode
Uric Acid
Serum

Urine





Joint Fluid

Crystals

1 ml serum. (SST)

30 ml aliquot of well-mixed 24-hour collection. Add 10 ml 5N NaOH at beginning of collection. Store at room temperature. Record 24-hour volume on requisition.

See Synovial Fluid Analysis.

See Synovial Fluid Analysis.

Female: 2.4-6.9 mg/dl
Male: 2.5-8.4 mg/dl

250-750 mg/24 hr.

Colorimetry – Timed endpoint

Colorimetry
Urinalysis
Complete

Without Microscopy Includes: pH Specific gravity, ketones, Protein glucose, blood, And bilirubin

10 ml aliquot of first morning urine.

10 ml aliquot of first morning urine.


See Addendum

See Addendum 

Microscopic Red Compensator

Reflectance Spectophotometer Refractometry
Urine Screen
(Bacteriuria)
See Culture, Urine.    
Uroporphyrins See Porphyrins.    
Valium See Diazepam.    
Valproic Acid (Depakene)

Blood
2 ml serum. Do not collect in SST. Therapeutic Range:
50-100 mg/ml
FPIA
Vanilmandelic Acid (VMA)

Urine.
75 ml aliquot of well-mixed 24-hour urine. Add 30 ml 6N HCL. Record 24-hour volume on requisition. 2.0-10.0-mg/24 hrs. Spectrophotometry
Varicella Antibody

Serum (Immune Status)
1 ml serum. Immune>0.9 E.U.
Not Immune<0.9 E.U.
EIA
Vasoactive Intestinal Polypeptide (VIP)

Plasma
3 ml EDTA plasma. Draw specimen in lavender top tube. Mix gently and chill in ice bath immediately. Separate plasma into 2 plastic vials. FREEZE. 0-50 pg/ml RIA
Vasopressin See Anti-Diuretic Hormone    
VDRL

Cerebrospinal Fluid
2 ml CSF. Non Reactive Agglutination
Viscosity

Serum
4 ml serum. 1.4-1.8 Rel. to H2O Viscometry
Vitamin A

Serum
2 ml serum. FREEZE in plastic vial. Wrap in aluminum foil to protect it from light. 30-80 mg/dl HPLC
Vitamin B1 (Thiamine)

Blood
EDTA whole blood frozen (Lavender top tube). Protect from light by wrapping in aluminum foil. 87-280ng/ml HPLC
Vitamin B12

Serum
2 ml serum. Fasting specimen preferred. 200 – 835 pg/ml ECLIA – Electro Chemiluminescence Immunoassay Competition Principle
Vitamin B6
(Pyridoxal-6-Phosphate)
Plasma
3 ml EDTA plasma (Lavender top Tube). FREEZE. Protect from light by wrapping in aluminum foil. 3.3-26.0ng/ml HPLC
Vitamin C
(Ascorbic Acid)
Serum
2 ml serum. FREEZE in plastic vial. Protect from light by wrapping in aluminum foil. 0.2-2.0 mg/dl Spectrophotometry
Vitamin D
(1, 25 dihydroxy)

Serum
5 ml serum. FREEZE. 19-67pg/ml RIA
Vitamin D
(25 Hydroxy)
Serum
2 ml serum. FREEZE 9-52ng/ml Competitive Protein Binding
Vitamin E (Tocopherol) 3 ml serum. FREEZE in plastic vial. Protect from light by wrapping in aluminum foil. Alpha Tocopherol: 5.7-19.9mg/l

Beta-Gamma Tocopherol: £4.3mg/l
HPLC
Vitamin K1 3ml frozen plasma (Green Top Tube). Protect from light by wrapping in aluminum foil. 80-1160pg/ml HPLC
Xylose Absorption Test
Urine















Blood

15 ml urine-Plastic container. Aliquot from a well-mixed, 5-hour collection. Refrigerate during collection and prior to delivery to laboratory. If specimen is to be stored for some time, add NaF (10 mg per mL of urine) as a preservative. Record total volume for 5-hour collection, and the amount of D-Xylose administered on both the specimen container and the test request form. Patient must be fasting before and during this test.

1 full gray top tube. Mix gently by inversion. Collect two hours after administering D-Xylose.

See Addendum










See Addendum

Spectrophotometry















Spectrophotometry
Zarontin See Ethosuximide.    
Zinc

Plasma
Use Kit 493 1 royal blue top tube. Mix gently by inversion. Separate plasma from cells within 2 hrs. 60-130 mg/dl Atomic Absorption
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