Test Name |
Submission Requirements |
Reference Range |
Methodology |
|---|---|---|---|
| syphilis Syphilis Serology (RPR) | 1 ml of serum or plasma | Negative | Rapid Plasma Reagin |
| 17-Hydroxy- progesterone. | 1ml serum. Morning specimen recommended. | Male: 3.0-220 ng/dl Female: Follicular: 20-100 ng/dl Luteal: 100-500 ng/dl Postmenopausal: <70 ng/dl |
RIA |
| 17-ketosteroids Urine, Total Urine, Fractionated |
15 ml aliquot of well-mixed 24-hour urine. Place 1 tablet of boric acid in container prior to collection. Note 24 hour volume on requisition. See Androsterone |
Male: 7-20 mg/24 hrs. Female: 5-15 mg/24 hrs. |
Modified Zimmerman Reaction |
| 3 Alpha Androstanediol Glucuronide | 2 ml serum. | Adult male: 2.6-16.0 ng/ml Adult female: 0.60-8.1 ng/ml |
Enzyme Digestion, Chromatography, RIA |
| 5’ Nucleotidase Serum |
2 ml serum. | 2-15 U/L | Spectrophotometry |
| 5-Hydroxyindole-acetic Acid (5-HIAA, Serotonin) urine, Quantitative |
10ml Aliquot of well mixed 24 hr. urine. Add 10 grams Boric Acid to container prior to collection. Note 24 hour volume on requisition. | <9.0 mg/24 hr. | HPLC or Immunoassay |
| 5'Nucleotidase | 2 ml of serum | 2-15 U/L | Spectrophotometry |
| ABO Antibody Titer |
One full pink top tube. Do not use SST tube. Label test tube with patient full name. | Not applicable | Gel technology |
| ABO Group and Rh Type | 1 pink top tube. (Do not use SST.) Label tube with patient's full name and MR# |
Not applicable | Gel technology |
| Acetylcholine Receptor Antibody Serum |
1 ml serum. Collect in plain red top tube. Indicate if sample is from immunosuppressed patient. | < 0.7 nmol | Radio Binding Assay |
| Acid Fast Bacilli Smear |
Slide or specimen. | No acid fast bacilli seen. | Acid Fast stain |
| Acid Hemolysins (Ham Test) |
1 lavender top tube, plus 1 ml FROZEN serum | 0% Lysis | Acid Serum Lysis |
| Acid Phosphatase, Prostatic (PAP) Serum |
2 ml serum. Place on ice immediately after collection. FREEZE | <2.5 mL |
Chemiluminescence |
| Acute Hepatitis Panel | See Test Profiles | ||
| Adenovirus Antibody | 1 ml serum Plain red top tube. Paired sera advised to detect rising titers. | <1:8 | Complement Fixation |
| Adrenocorticotrophic Hormone (ACTH) Plasma |
2 ml EDTA plasma. Draw in prechilled lavender top tube. Draw between 7am and 10am. Separate plasma and freeze in plastic vial. | <70 pg/ml | Chemilluminescence |
| Alanine Amino Transferase (ALT, SGPT) | 1 ml serum (SST) | 10-60 U/L | Kinetic Assay |
| Albumin Serum |
1 ml serum (SST) | Female: 3.6- 4.5 g/dl Male: 3.5 -5.2 g/dl |
Colorimetry endpoint Timed endpoint |
| Alcohol, Ethyl Serum |
2 ml serum | < 1.0 mg/dl | Enzymatic |
| Aldolase Serum |
2 ml frozen serum. Separate serum and freeze in plastic vial. | < 8.0 U/L | Enzymatic |
| Aldosterone Serum |
2 ml serum. Draw in plain red top tube. Separate from cells within 30 minutes. FREEZE. | Sodium intake 100-200 mEq/l Supine: 2-9 ng/dl Upright: 3-35 ng/dl 2.3-21.0-mcg/24 hr. (Normal sodium intake) |
Extraction, chromatography, RIA Hydrolysis, extraction, RIA |
| Alkaline Phosphatase, Leukocyte (LAP) | 1 green top tube.20-100 cell score | 20-100 cell score | Cytochemistry |
| Alkaline Phosphatase Serum |
1ml serum (SST) | 26-88 U/L | Kinetic |
| Allergy Testing Rast Allergens |
2ml serum (SST) per group of 6 allergens. REFRIGERATE. Specify number of and types of allergens on requisition. | See report for interpretation See addendum for list of Allergens available. |
FEIA |
| Alpha-1-Antitrypsin Serum, Total |
1ml serum. Draw in plain red top tube. | 83-199 mg/dl | Nephelometry |
| Alpha-Fetoprotein (AFP) Amniotic Fluid, Quantitative |
2 ml amniotic fluid | See report for interpretation. | Chemiluminescence |
| Alpha-Fetoprotein (AFP) Maternal Serum, Quantitative |
2 ml serum | See report for interpretation | Chemiluminescence |
| Alpha-Fetoprotein (AFP) Tumor Marker, serum |
2 ml serum | < 5.75 U/ml | Chemiluminescence |
| Alpha-galactosidase A | 3 Green top tubes (peripheral blood). DO NOT FREEZE. Submit to lab within 24 hours of collection. Submit with a completed genetic requisition form | 0.016-0.20 U/L | Fluorometric |
| Aluminum Plasma |
2 ml Heparinized Plasma-Dark blue top trace metal tube. Obtain KIT 493 from lab. Transfer plasma within 2 hours to aluminum-free plastic vial (included in KIT 493). | 3-10 mcg/l | Atomic Absorption |
| Aluminum Serum |
2mL serum- dark blue top tube. Obtain KIT 492 from lab. Transfer serum within 2 hours to aluminum free plastic vial (included in KIT 492). | 3-10 mcg/l | Atomic Absorption |
| Amikacin | 2 ml serum. Collect in plain red top tube. Do not collect in SST | Trough 0-10 us/ml Peak 10-30 us/ml |
EIA Toxic > 35 us/ml |
| Amino Acid Fractionation Plasma, Quantitative |
2ml heparinized plasma (green top tube). Separate from cells immediately and place in plastic vial. FREEZE. Patient age and sex required. | See report | Chromatography (HPLC) |
| Amino Acid Fractionation Urine. Quantitative |
10ml aliquot of well-mixed 24-hour urine. Acidify to pH 3-5 with 6N HCI. Refrigerate while collecting. Note 24 hr. volume on requisition. Indicate patient’s age and sex. FREEZE. | See report | Chromatography (HPLC) |
| Amino Acid Screen Plasma |
3-5ml heparinized plasma (green top tube). FREEZE plasma in plastic vial. | No Abnormal pattern detected. | Chromatography (HPLC) |
| Amino Acid Screen Urine |
10ml frozen random urine. | No abnormal pattern detected. | Chromatography (HPLC) |
| Aminolevulinic Acid (ALA) | 2ml aliquot of refrigerated well mixed 24 hr. urine. FREEZE. Protect from light by wrapping with aluminum foil. Acidify aliquot with 2 drops 6N HCL. Record total volume on Requisition. Do not acidify if requested in combination with urine lead. | 6.4 mg/24hrs or less. | Colorimetric |
| Amiodarone and Metabolite Serum | 3ml serum from plain red top tube. Do not collect in SST. FREEZE. Collect no sooner than 12 hrs. after last dose. | Therapeutic Range: 1.5-2.5 mg/l | Chromatography (HPLC) |
| Amitriptyline and Nortriptyline (Elavil and Travail) | 2ml serum from plain red top tube only. Do not collect in SST. Collect no sooner than 12 hrs after last dose. | Therapeutic Range: 125-150mcg/l | Chromatography (HPLC) |
| Ammonia Plasma |
3ml heparinized (green top tube) plasma. Send immediately on ice or FREEZE. | 11-35 mmol/l (No normal range) | Enzymatic Timed endpoint |
| Amoebiasis Purged Stool |
Submit stool sample in LV-PVA Fixative container. | No ova or parasites found | Microscopy |
| Amphetamine Serum |
5ml serum. Collect in plain red top tube. | Negative | GC/MS |
| Amphetamine Urine |
10ml random urine. | Negative | EIA |
| Amylase Isoenzymes Serum ( includes total amylase |
2mL serum. FREEZE serum in plastic vial. | See lab report | Enzymatic- Column Chromatography |
| Amylase Serum Urine Fluid |
1ml serum (SST) 10ml Aliquot of refrigerated 24-hour urine. Record total volume on requisition 3ml fluid |
44-128 U/L 24-408 U/24 hrs. Variable |
Enzymatic Enzymatic Enzymatic |
| Androstenedione Serum |
2ml serum. Collect in plain red top tube. FREEZE serum in plastic vial. | See Addendum. |
EXTRACTION, RIA |
| Angiotensin-1- Converting Enzyme Serum |
1ml serum. Collect in plain red top tube. |
8-52 U/L | Spectrophometry (Kinetic) |
| Anti-A, Anti-B Titers | See ABO Antibody Titer. | ||
| Anti-Adrenal Cortex Antibodies Serum |
2ml serum. Refrigerate. | None detected. | IFA |
| Antibiotic Sensitivity | Performed automatically on all relevant findings. | N/A | Disc Method a/o Breakpoint |
| Antibody Identification and Titer | 1 pink top tube. Do not use SST tube. Label test tube with patient’s full name. |
N/A | Gel technology |
| Antibody Screen Red Cell (does not include identification and titer.) Phenotyping |
1 pink top tube. Do not use SST tube. Label tube with patient’s full name. |
Gel technology | |
| Antibody Screen with Reflex to identification and Titer. (No specimen will be saved for comparative titers.) |
1 pink top tube. Do not use SST tube. Label test tube with patient’s full name. |
Negative | Gel technology |
| Antibody Screen (Pregnancy Monitoring) |
1 pink top tube. Do not use SST tube..Physician must provide mother’s date of birth.* Label test tube with patient’s full name. |
Negative | Gel technology |
| Anti-Cardiolipin Antibodies, lgA,lgG and lgM serum |
2ml serum. Collect in plain red top tube. |
< 10 SDU | EIA |
| Anti-Convulsant Levels | See individual drug listings | ||
| Anti-Diuretic Hormone (Vasopressin) Plasma |
4ml Frozen EDTA plasma (lavender top tube). Draw into pre-chilled lavender top tubes. Separate plasma immediately into plastic vial and freeze. | 1.0 – 13.3 pg/ml | Extraction, RIA |
| Anti-DNA ( ds ) Antibodies Serum |
2 ml serum. | <30 U/ml | EIA |
| Anti-ENA (SSA/SSB, SM & RNP) Panel Serum |
2ml serum. Collect in plain red top tube. |
Negative | EIA |
| Anti-Glomerular Basement membrane Antibodies (lgG) Serum |
2ml serum. Collect in plain red top tube. |
<5 units | Immunoassay |
| Anti-Islet Cell Antibody Serum |
2ml serum. Collect in plain red top tube. |
None detected | IFA |
| Anti-Mitochondrial Antibodies Serum |
2ml serum. | <1:10 | IFA |
| Anti-Myocardial Antibodies Serum |
2ml serum. Collect in plain red top tube. |
None detected | IFA |
| Anti-Nuclear Antibody Serum |
1 ml serum. | Negative | EIA |
| Anti-Parietal Cell Antibodies Serum |
2 ml serum. | <1:20 | IFA |
| Anti-Platelet Antibody Serum |
2 ml serum. Collect in plain red top tube. |
None detected | Flow Cytometry |
| Anti-Reticulin Antibodies Serum |
2 ml serum. Collect in plain red top tube. |
Negative | IFA |
| Anti-Smooth Muscle Antibodies Serum |
2 ml serum. | <1:20 | IFA |
| Anti-Streptolysin 0 Titer | 2 ml serum. | <200 IU/ml | Nephelometry |
| Anti-Striated (Skeletal) Muscle Antibodies serum |
2 ml serum. Collect in plain red top tube. |
None detected | IFA |
| Anti-Thrombin III Activity | One blue top tube. Separate plasma from cells immediately. Transfer plasma to plastic vial and FREEZE. |
78-115% of normal | Chromogenic |
| Anti-Thyroid Antibodies (Thyroglobulin and Peroxidase) |
2 ml serum. | Thyroglobulin Ab: <1.0 U/ml Peroxidase Ab: <0.3 U/ml |
Chemiluminescence |
| Arsenic Blood Urine |
1 royal blue top tube (kit-493). Order KIT 493 from the lab. 7 ml aliquot of well mixed 24 hr urine. Add 20 ml 6N HCI to collection container prior to start of collection. Record total volume. |
< 3 mcg/dl <80 mcg/l |
Mass Spectrometry Mass Spectrometry |
| Ascorbic Acid | See Vitamin C | ||
| ASO | See Anti-Streptolysin 0 Titer. | ||
| Aspartate Amino Transferase (AST, SGOT) Serum |
1 ml serum (SST) | 10-42 U/L | Enzymatic |
| Aspergillus Antibodies Serum Screen Titer |
1 ml serum. Collect in plain red top tube. Paired sera advisable to detect rising titers. |
None Detected <1:8 |
Immunodiffusion |
| Australian Antigen | See Hepatitis B Surface Antigen | ||
| Babesia Screen | 1 lavender top tube. | None seen. | Microscopic Examination |
| Bacterial Antigens H. Influenza, type b N. Meningitidis group ACY w135 N. Meningitidis group B S. Agalactiae (group B) S. Pneumoniae E. Coli K1 |
2 ml CSF, 2 ml serum or 10 ml random urine in sterile vial. | Negative | Latex Agglutination |
| Barbiturates Blood |
3 ml serum. Collect in plain red top tube. |
See Addendum |
GC and FPIA |
| Barr Bodies Buccal Smear |
Smear of Buccal Mucosa fixed in 95% alcohol. | N/A | See page 4 for further instructions. |
| Bence Jones Protein | See Immunoelectrophoresis, Urine | ||
| Benzodiazepines Serum Urine |
3 ml serum. Collect in plain red top tube. 25 ml random urine |
Negative Negative |
HPLC GC/MS |
| Beta-2 Microglobulin Serum |
1 ml serum | <3.0 mg/l | Nephelometry |
| Beta-HCG | See Chorionic Gonadotropin (HCG). | ||
| Bilirubin Serum, Total Serum, Direct Serum, Indirect |
1 ml serum. (SST) 1 ml serum. (SST) 1 ml serum. (SST) |
0D 0-5.8 mg/dl 1D 0-8.2 mg/dl 5D 0.2-1.0 mg/dl 0.1-0.3 mg/dl 0.0-0.9 mg/dl |
Colorimetry Colorimetry Calculated |
| Blastomyces Antibodies Serum (Screen) |
2 ml serum. Collect in plain red top tube. |
None detected | Immunodiffusion |
| Bleeding Time, Ivy | Please call Coagulation lab for appt. (670-1085) and instructions. | 1-7 min. | Functional |
| Blood Group and Rh Type | See ABO Group and Rh Type. | ||
| Blood Urea Nitrogen | See Urea Nitrogen | ||
| Brain Natriuretic Peptide B-Type Natriuretic Peptide |
5 ml frozen plasma from pre-chilled lavender top tube. Patient should be recumbent for 20 minutes prior to drawing blood. Place tube in ice and transport to lab. | See Addendum |
RIA |
| Bromide Serum |
3 ml serum. | See Addendum. |
Spectrophotometry |
| Brucella Agglutination Serum |
1 ml serum. Paired sera advisable to detect rising titers. |
<1:80 | Agglutination |
| C-1 Esterase Inhibitor Serum |
1 ml serum. Collect in plain red top tube. Separate and FREEZE immediately in plastic vial. |
>68 % | Nephelometry |
| C1Q Binding Serum |
2 ml serum. Collect in plain red top tube. Separate serum and FREEZE in plastic vial. |
0-13% Normal 13-16% Borderline >16% Elevated |
Radial Immunodiffusion |
| CA125 | 1 ml serum. | 0.6 – 35.0 U/ml | Chemiluminescence |
| CA15-3 - Sendout | 1 ml serum. Collect in plain red top tube. |
< 32 U/ml. | Chemiluminescence |
| CA19-9 - Sendout | 2 ml serum. Collect in plain red top tube. |
< 37 U/ml | Chemiluminescence |
| Calcitonin Serum |
1 ml frozen serum. Collect in plain red top tube. Separate serum and FREEZE in plastic vial. |
Male: <8 pg/ml Female: <4 pg/ml |
Chemiluminescence |
| Calcium Serum, Total Serum, Ionized Urine |
2 ml serum. (SST) 1 SST tube. Do not open. 50 ml aliquot of well mixed 24 hr. urine. Add 10 ml 6N HCI at beginning of 24 hr collection. Note 24 hr. volume on requisition. |
8.5-10.5 mg/dl 1.12 - 1.320 mmol/L 100-300 mg/24° No range for random |
Ion Specific Electrode Ion Specific Electrode Ion Specific Electrode |
| Calculus Analysis | Submit specimen in a clean, sealed container. | See report. | Infrared Spectroscopy and X-Ray |
| Candida precipitins | 10 ml serum. Collect in plain red top tube. |
None detected | Immunodiffusion |
| Carbamazepine | 2 ml serum. Do not collect in SST. |
Therapeutic Range: 8-12 mcg/mL. | FPIA |
| Carbon Dioxide (CO2) Serum |
2 ml serum (SST) | 24-31 mmol/l | Ion Selective Electrode |
| Carbon Monoxide (Carboxyhemoglobin) blood |
ABG Syringe |
0-2% | Co-Oximetry |
| Carcinoembryonic Antigen (CEA) | 2 ml serum. | 0 –5 ng/ml | ECLIA Sandwich Principle |
| Cardiolipin Antibody (IGA) | 2 ml serum. Collect in plain red top tube. |
Negative <12 APL Equivocal 12-15 APL Elevated >15 APL |
ELISA |
| Cardiolipin Antibody (IGG) | 2 ml serum. Collect in plain red top tube. |
Negative <12 APL Equivocal 12-15 APL Elevated >15 APL |
EIA |
| Cardiolipin Antibody (IGM) | 2 ml serum. Collect in plain red top tube. |
Negative <12 APL Equivocal 12-15 APL Elevated >15 APL |
EIA |
| Cardiovascular Evaluation | See test Profiles | ||
| Carotene Serum |
2 ml serum. Collect in plain red top tube. Wrap in aluminum foil to protect from light. |
50-300 mcg/dl | HPLC |
| Catecholamines Fractionated, Serum - Norepinephrine - Epinephrine - Dopamine - Total catecholamines |
2 pre-chilled green top tubes or 5 ml heparinized plasma. FREEZE. (Submit specimen on ice). | See Addendum |
HPLC |
| Catecholamines, Fractionated, Urine - Norepinephrine - Epinephrine - Dopamine |
25 ml aliquot of well-mixed 24-hour urine. Separate serum and FREEZE in plastic vial. Add 25 ml 6N HCL to container at start of collection. Record total volume on both specimen container and request form. | See Addendum |
HPLC |
| Cell Count Fluid | 2 ml fluid. Submit fluid in lavender top tube. |
N/A | Chamber Counts Cytoprep |
| Celontin | See Methsuximide | ||
| Ceruloplasmin Serum |
1 ml serum. | 18-55 mg/dl. | Nephelometry |
| Chlamydia | Swab in M4 Transport Media or urine | Negative | PCR |
| Chloride Cerebrospinal fluid Serum Urine |
1 ml CSF. 1 ml serum. (SST) 50 ml aliquot of well mixed 24 hr. urine. Note 24 hr. volume on requisition. |
122-132mmol/l 95-108mmol/l 110-250mmol/24 hr. |
Ion Selective Electrode Ion Selective Electrode Ion Selective Electrode |
| Chlorpromazine serum |
See Thorazine | ||
| Cholesterol Total, Serum High Density Lipoprotein (HDL) |
1 ml serum. (SST) 3 m serum. (SST) Fasting specimen required. |
120 – 200 mg/dl (Chart) |
Timed endpoint. Enzymatic Direct Measurement |
| Cholesterol Low Density Lipoprotein (LDL) |
3 ml serum. Fasting specimen required. |
See Addendum. |
Calculation |
| Cholinesterase Plasma |
See Pseudocholinesterase | ||
| Cholylglycine (Bile Acid) Serum |
3 ml frozen serum. Collect in plain red top tube. Transfer serum to plastic vial and FREEZE. Fasting specimen required. | Fasting: <68 mcg/dl Postprandial: Approx. 3x fasting |
RIA |
| Chorionic Gonadotropin Urine, Qualitative Pregnancy Serum, Qualitative Pregnancy Serum, Quantitative Pregnancy |
10 ml first morning urine. 2 ml serum. 2 ml serum. |
Negative Negative 0-4.9 mIU/ml |
Rapid Chromatographic Immunoassay Rapid Chromatographic Immunoassay ECLIA – Sandwich Principle |
| Chromium Serum |
2ml serum- dark blue top tube. Obtain KIT 492 from lab. Transfer serum within 2 hours to aluminum free plastic vial (included in KIT 492). |
£ 2.0 ng/dl | Atomic Absorption |
| Citric Acid Urine |
10 ml aliquot from a well mixed 24 hr urine collection. Add 10 gm Boric Acid to container at start of collection. | Female: 125-900 mg/24 hr. Male: 65-650 mg/24 hr. |
Spectrophotometry |
| Clonazepam | 3 ml frozen serum. Collect in plain red top tube. Transfer serum to plastic vial and FREEZE. |
Therapeutic Range: 30-60 ng/ml Partially Toxic: >100 ng/ml |
HPLC |
| Clostridium Difficile Toxin A/B Assay Stool |
Submit stool specimen in sterile container. Specimens accepted Mon-Fri only. | Negative | ELISA |
| CMV | See Cytomegalovirus titer. | ||
| Coccidioidomycosis Serum(Screen) |
2 ml serum. Collect in plain red top tube. |
None detected | Immunodiffusion |
| Cold Hemagglutinins | 2 ml serum. Clot at 37ºC and separate serum from cells. DO NOT REFRIGERATE. | <1:40 | Agglutination |
| Complement C1Q Component Serum |
2 ml frozen serum. Collect in plain red top tube. Transfer to plastic vial and FREEZE. |
14-20 mg/dl. | Radial Immunodiffusion |
| Complement C2 Serum |
2 ml frozen serum. Collect in plain red top tube. Transfer to plastic vial and FREEZE. |
1.6-3.6 mg/dl | Radial Immunodiffusion |
| Complement C3 Serum |
1 ml frozen serum. Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE. |
83-177 mg/dl | Nephelometry |
| Complement C4 Serum |
2ml frozen serum. Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE. |
15-45 mg/dl | Nephelometry |
| Complement C5 Serum |
2 ml serum. Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE. |
7.3-19.0 mg/dl | Radial Immunodiffusion Female: 9-153 ng/ml |
| Complement C6 Serum |
2 ml frozen serum. Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE. |
3.6-7.5 mg/dl | Hemolytic Assay |
| Complement Specific Immune Complex Serum |
2 ml frozen serum. Collect in plain red top tube. Transfer plasma to plastic vial and FREEZE. |
Normal: <15 mEq/ml Borderline: 16-19 mEq/ml Elevated: >20 mEq/ml |
EIA |
| Complete Blood Count With Differential Without differential |
1 lavender top tube. 1 lavender top tube. |
See Addendum. See Addendum. |
Particle counter, Flow Cytometry and Microscopy Particle counter. |
| Coombs, Direct Blood |
1 lavender top tube. | Negative | Immune Agglutination |
| Copper Serum Urine |
2 ml serum. Collect using Kit-#492. Order KIT-492 from lab. 30 ml aliquot of well-mixed 24-hour urine. Indicate 24 hr. volume on requisition. MUST use special acid washed container and transfer vial (available through the lab). |
70-155 mcg/dl 15-50 mcg/24 hr. |
Atomic Absorption Atomic Absorption |
| Cortisol Free, Urine Serum Cortisol |
75 ml aliquot of well mixed 24 hr. urine. Add boric acid tablets at start of collection Note 24 hr. volume on requisition. FREEZE. 1 ml serum. |
20 – 90mcg/24 hr. 2.3 - 19.4 ng/dl mg/dl |
RIA Chemiluminescence |
| Coxsackie A Virus Antibodies (2,7,10,16) | 2 ml serum. Collect in plain red top tube. Paired sera advisable to detect rising titers. |
No antibody detected | Complement Fixation |
| Coxsackie Virus Antibody (B1-B6) | 2 ml serum. Collect in plain red top tube. Fasting. Paired sera advisable to detect rising titers. |
<1:8 | Complement Fixation |
| C-Peptide Serum |
2 ml serum. Collect in plain red top tube. Separate serum and FREEZE in plastic vial. Fasting sample required. |
0.8-3.1 ng/ml | Immunochemiluminometric |
| C-Reactive Protein (CRP) Serum |
2 ml serum. | Negative | Nephelometry |
| Creatine Kinase Serum, Total (CK) CK-MB Includes Total CK |
2 ml serum (SST) 2 ml serum. FREEZE in plastic vial if not deliverable within 24 hours. |
22-269 U/L 0-7 mcg/l |
Enzymatic Chemiluminescence |
| Creatinine Clearance | 2 ml serum and 10 ml Aliquot of well-mixed 24-hour urine specimen. Note 24 hr. volume on requisition. | 60-150 ml /min | Jaffe Rate |
| Creatinine Serum Urine |
2 m. serum. (SST) 25 mL aliquot of well mixed 24 hr. urine. Note 24 hr. volume on requisition. |
0.5-1.5 mg/dl 1-2 gm/ 24 hrs |
Jaffe Rate Jaffe Rate |
| Cryoglobulins Serum, Qualitative |
2 ml serum. Allow blood to clot at 37?C and separate serum. DO NOT REFRIGERATE, Transport at room temperature. |
Negative | Cold Precipitation |
| Cryptococcus Antigen Serum CSF |
1 ml serum. 1 ml CSF. |
Negative Negative |
Latex agglutination Latex agglutination |
| Culture T. Vaginalis |
10 ml random urine | No T. vaginalis recovered | |
| Culture Throat for Beta-Strep only |
Swab in transport media. | Negative for beta strep | |
| Culture Urine |
Full sterile culture tube or sterile jar. | No growth. | |
| Culture Viral Isolation |
Virus isolation transport media | No growth | |
| Culture Wound |
Swab in transport media | No growth | |
| Cultures Acid Fast CSF |
1 ml CSF in sterile tube. |
No AFB recovered. |
|
| Cultures Acid Fast Gastric |
Gastric sample in sterile container. |
No AFB recovered. | |
| Cultures Acid Fast Misc. |
Submit sample in sterile container | No AFB recovered | |
| Cultures Acid Fast Sputum |
Sputum in sterile container. | No AFB recovered | |
| Cultures Acid Fast Urine |
40 ml urine in sterile container. | No AFB receovered | |
| Cultures Anaerobes |
Swab in Anaerobic Transport medium. |
No growth |
|
| Cultures B. Pertussis |
Submit naso-pharyngeal culture on 2 Calgiswabs or cough plate |
No B. Pertussis isolated |
|
| Cultures Blood |
5 ml blood in each of two-blood culture set bottles. Indicate diagnosis, antibiotic therapy, date and hour drawn on requisition. |
No growth |
|
| Cultures Body Fluid |
Submit fluid in sterile container. |
No growth. |
|
| Cultures Cerebrospinal Fluid |
Submit CSF in sterile container. | No growth | |
| Cultures Chlamydia |
Submit swab in M4 Medium or first morning urine. | Negative | |
| Cultures Cryptosporidium |
Stool | Negative | |
| Cultures Fungi |
Submit material in sterile container or swab in transport media | No growth | |
| Cultures Genital |
Swab in transport media. | No growth | |
| Cultures Gonorrhea |
Swab in Amies charcoal media. | No N. gonorrhea recovered. | |
| Cultures Gonorrhea PCR |
M4 or urine for male only Endocervical | Negative | |
| Cultures Herpes Simplex |
Swab in viral transport media (M4). | No growth. | |
| Cultures Misc. |
Swab in transport media. | No growth | |
| Cultures Mycoplasma |
Swab in Mycoplasma transport media | No mycoplasma recovered. | |
| Cultures Nose/Respiratory |
Swab in transport media | Normal mouth bacteria | |
| Cultures Sputum |
Sputum in "Sealtight" container. | Normal mouth bacteria | |
| Cultures Stool |
Stool in Cary and Blair media (yellow top container) | No enteric pathogens isolated | |
| Cultures Throat |
Swab in transport media. | Negative for beta strep | |
| Cultures Acid Fast Blood |
Note: All culture samples must be submitted according to instructions on page5. 10 ml blood in Dupont Isolator. |
No AFB recovered. |
|
| Cyanide Whole blood |
2 gray top tubes. | <0.1 mg/dl | Gas Diffusion, Colorimetric |
| Cyclic Amp Plasma Urine |
3 ml of EDTA plasma (lavender top tube). Separate plasma within 15 minutes of collection. Transfer plasma to plastic vial and FREEZE. 10 ml random urine. Acidify aliquot with 2 ml 6N HCL to keep pH below 3. FREEZE. |
3.9 – 13.1 nmol/l 1.0 – 11.5 nmol/l |
RIA RIA |
| Cystine Urine, Quantitative |
25 ml aliquot of well-mixed 24-hour urine. Refrigerate while collecting. Acidify aliquot with 6N HCL to pH 2-3. Note 24-hour volume on requisition. Ship frozen. | 10-100 mg/24 hr. | HPLC |
| Cytgology Smears |
Slides fixed in 95% alcohol. | N/A | |
| Cytology ThinPrep | Specimen submitted in ThinPrep vial | N/A Positive ASCUS reflexed to HPV | |
| Cytology Buccal Smear |
Smear of buccal mucosa fixed in 95% alcohol. | N/A | |
| Cytology Endometrial Wash |
Specimen in equal volume of 50% alcohol. | N/A | |
| Cytology Gynecological (Includes Hormonal Evaluation) |
Smear. DO NOT ALLOW SMEAR TO DRY. Spray smear with fixative or place slide into 95% alcohol. | N/A | |
| Cytology Rffusion, CSF |
Specimen in equal volume of 50% alcohol. | N/A | |
| Cytology Sputum |
Specimen in 50% alcohol. | N/A | |
| Cytology Urine |
Submit specimen in equal volume of 50% alcohol. | N/A | |
| Cytology Aspirate fluid Brushings |
Note: Cytology specimens must be submitted according to instructions on Page 4. Specimen in equal volume of 50% alcohol Smear. DO NOT ALLOW SMEAR TO DRY. Spray smear with fixative or place slide into 95% alcohol. |
N/A N/A |
|
| Cytomegalovirus AB IgM | 1 ml serum | Negative | ELISA |
| D-Dimer Assay Plasma |
D-Dimer Assay Plasma 2 ml Plasma. Collect in blue top tube. |
Cut off value <209 ng/ml | Turbidimetric Immunoassay |
| Dehydroepiandrosterone (DHEA) Unconjugated |
1 ml serum. Collect in plain red top tube. |
Female: 130-980 Male: 180-1250 ng/dl |
RIA |
| Dehydro-epiandrosterone Sulfate (DHEA-S) | 1 ml serum. | See Addendum |
Chemiluminescence |
| Delta Virus Antibody IGG IGM |
2 ml serum | Negative Negative |
EIA |
| Desipramine Serum |
2ml serum. Collect in plain red top tube. Do not collect in SST. |
Therapeutic Range: 50-300 mcg/l Potentially toxic: >500 mcg/l |
HPLC |
| Diazepam (Valium) Serum |
3 ml serum. Collect in plain red top tube. Do not collect in SST. |
Therapeutic Range: 0.5-2.0 mg/l Potentially toxic: >3.0 mg/l |
HPLC |
| Dibucaine Inhibition | See Pseudocholinesterase | ||
| Digitoxin (Digitalis) Serum |
1 ml serum. Collect in plain red top tube. Do not collect in SST. |
Therapeutic Range: 10-30 mcg/l Potentially toxic: >30 mcg/l |
RIA |
| Digoxin Serum |
2 ml serum. Do not collect in SST |
Therapeutic Range: 0.9-2.0 ng/ml | Inverse Immunoturbidity |
| Dilantin | See Phenytoin | ||
| Direct Coombs Blood |
See Coombs, Direct, Blood. | ||
| Doriden (Glutathimide) Serum |
3 ml serum. Collect in plain red top tube. Do not collect in SST tube. |
Therapeutic Range: 3.0-7.0 mg/l | GC |
| Drug Screen, Comprehensive Blood Gastric Urine |
5 ml serum or 1 green top tube. Do not use SST. 10 ml gastric contents. 50 ml random urine. |
None detected Negative Negative |
Gas chromatography Chromatography MEIA |
| D-Xylose | See Xylose Absorption. | ||
| Echinococcus Antibody | 2 ml serum. Fasting specimen required. Paired sera advisable. |
Negative | ELISA |
| Elavil | See Amitriptyline | ||
| Electrolyte Panel | See Test Profiles. | ||
| Electrophoresis | See individual listings | ||
| Enterobius Vermicularis (Pinworm) | Pinworm Paddle or scotch tape | Negative | Microscopic Examination |
| Eosinophil Count Nasal Smear Blood |
Send air-dried smear on slide labeled with patient’s name. 1 lavender top tube. |
0% 50-400 cells/cmm |
Cytochemistry Microscopic Examination |
| Epstein-Barr Virus Ab. Screen (Capsid IgG and IgM, Nuclear Antigen IgG) | 2 ml serum. Specify age of patient on request form. | See lab report. | ELISA |
| Epstein-Barr Virus, DNA by PCR | 2 ml EDTA plasma from lavender top tube | < 200 copies/ml | PCR |
| Erythrocyte Sedimentation Rate | See Sedimentation Rate, Erythrocyte. | Westergreen Excyte (Automated) | |
| Erythropoietin Serum |
2 ml serum. | >16 years 4.0 –19.5 mU/ml | Chemiluminescence |
| Estradiol Serum |
2 ml serum. | See Addendum |
Chemiluminescence |
| Estriol Serum |
1 ml serum. | See Addendum. |
RIA |
| Estrogen Fractionated Includes Estrone and Estradiol Serum Urine |
2 ml FROZEN serum. 100 ml aliquot of 24-hour urine. Add 10 grams boric acid at start of collection. Record total volume on requisition. |
See Addendum. see lab report. |
RIA RIA |
| Estrone Serum |
4.0 ml frozen serum. Freeze in plastic vial. Indicate patient’s age and sex on requisition. |
See Addendum. |
RIA |
| Ethanol | See Alcohol, Ethyl | ||
| Ethosuximide (Zarontin) | 3 ml serum. Collect in plain red top tube. Do not collect in SST. |
Therapeutic Range: 40-100 mg/l | RIA |
| Factor II Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection CSEPT - OUT |
| Factor IX Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor V Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor VII Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor VIII Antigen | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Electroimmunodiffusion |
| Factor VIII Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor X Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor XI Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor XII Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | 50-150% | Photo-optical Clot Detection |
| Factor XIII Assay | One blue top tube (sodium citrate). Tube must be completely filled to prevent anticoagulant dilution error. Invert tube six times immediately after drawing. Separate and FREEZE plasma immediately in plastic vial. | Present | Solubility |
| Fat, Stool | See Lipids, Stool | ||
| Ferritin Serum |
2 ml serum | Female: 10-120 ng/ml Male: 20-250 ng/ml |
ECLIA – Electro Immunoassay Sandwich Principle |
| Fetal Erythrocytes | See Kleihauer-Betke, Under Hemoglobin, Fetal. | ||
| Fetal Hemoglobin | See Hemoglobin, Fetal. | ||
| Fibrinogen Plasma, Quantitative |
1 blue top tube. Gently mix by inversion. |
200 - 400 | Photo-optical Clot Detection |
| Folic Acid Serum |
2 ml serum. Fasting specimen preferred. |
4.2-19.9 ng/ml Age Dependent |
ECLIA Electro Chemiluminescence Competition Principle |
| Follicle stimulating Hormone (FSH) Serum |
3 ml serum. | See Addendum. |
MEIA |
| Francisella Tularensis Agglutination Serum |
2 ml serum. | <1:80 | Agglutination |
| Free Erythrocyte Protoporphyrins (FEP) | 1 lavender top tube. Gently mix by inversion. |
<35 mcg/dl | Fluorometry |
| Free Fatty Acids Serum |
2 ml EDTA plasma (Lavender top tube). Separate plasma and FREEZE in plastic vial. | Adult 0.07-0.88 mmol/l | Enzymatic Spectrophotometer |
| Free Testosterone | See Testosterone, Free. | ||
| Free Thyroxine (Free T-4) |
3 ml serum. | 0.73-1.70 ng/dl. | ECLIA – Competition Principle |
| Free Thyroxine Index (T7) |
See Thyroxine Index, Free | ||
| G6PD, RBC | 1 full lavender top tube | 4.6 – 13.5 U/g HGB | Enzymatic |
| Gamma Glutamyl Transferase (GGT) | 1 ml serum. (SST) | 0-130 U/L | Enzymatic |
| Gastrin Serum |
2 ml serum. FREEZE in plastic vial. Fasting for 12 hours is required. |
<100 pg/ml | RIA |
| Gentamicin Serum |
2 ml serum. Do not collect in SST. |
Trough 0-10 un/ml Peak 10-12 un/ml |
Particle Enhanced Turbidimetric Inhibition Immunoassay |
| Glucose Tolerance 3 hour Includes: Fasting, _, 1, 2 and 3 hours. Blood and Urine | 5 gray top tubes. Indicate time on each tube and 5 urine samples. Gently mix tubes by inversion. | N/A | Oxygen Electrode |
| Glucose Tolerance, 6 hour Includes: Fasting, _, 1, 2, 3, 4, 5 and 6 hours. Blood and Urine | 8 gray top tubes. Indicate time on each tube and 8 urine samples. Gently mix tubes by inversion. | N/A | Oxygen Electrode |
| Glucose Blood, 2 hour Post Prandial Blood, Fasting Plasma Plasma, serum Fluid CSF, Quantitative Urine, Quantitative |
1 gray top tube. Indicate 2 hr post Prandial. Gently mix. 1 gray top tube. Gently mix. 3 ml serum. (SST) Submit fluid in gray top tube. 1 ml CSF. 25 ml Aliquot of well-mixed 24-hour urine. Add 5 ml Glacial acetic acid at beginning of 24 hr. collection. Note 24 hr. volume on requisition. |
70-140 mg/dl 70-115 mg/dl 70-115 mg/dl Variable 40-80 mg/dl 0-0.3 g/24hrs (No range for random) |
Oxygen Electrode Oxygen Electrode Oxygen Electrode Oxygen Electrode Oxygen Electrode Oxygen Electrode |
| Glycohemoglobin (Hemoglobin A1c) | 1 lavender top tube. | 5.5-9.0 % A1c | Liquid Chromatography HPLC |
| Gonadotropin, Chorionic | See Chorionic Gonadotropin. | ||
| Gram Stain for Gonococcus (Males only) |
Submit smear. Label with Patient’s name. | Negative for GC | Gram stain |
| Gram Stain Smear | Submit smear. Label with Patient’s name. | No organisms seen | Gram stain |
| Growth Hormone | 2 ml serum. | Adult: <8 ng/ml Child: <40 ng/ml |
Chemiluminescence |
| Guaiac, Stool | See Occult Blood, Stool. | ||
| Haptoglobin | 2 ml serum. | 50-170 mg/dl. | Nephelometry |
| HCG | See Chorionic Gonadotropin. | ||
| HDL Cholesterol | See Cholesterol HDL. | ||
| Heavy Metal Screen, Urine. Includes: Arsenic Lead Mercury |
90 ml aliquot of well mixed 24 hour urine collection (Acidify to pH of 2 with 20 ml 6N HCL) Record total volume on requisition. | Arsenic: <80 mcg/dl Lead: <50 mcg/dl Mercury: up to 20 mcg/dl |
Atomic Absorption Atomic Absorption Atomic Absorption |
| Helicobacter Pylori Ab. | One red top tube | Negative | EIA |
| Hemoglobin A1c | See Glycohemoglobin. | ||
| Hemoglobin S, Screen | See Sickle Cell. | ||
| Hemoglobin Variant | 1 lavender top tube. Invert several times. |
See Addendum |
Ion exchange HPLC |
| Hemoglobin, Fetal Kleihauer-Betke Qualitative Quantitative (Fetal Erythrocytes) |
1 lavender top tube. Invert several times. 1 lavender top tube. Invert several times. |
0-3% 0-3% Fetal |
Acid elution-Smears Acid elution-Smears |
| Hemoglobin, Unstable | 1 lavender top tube. | Negative | Isopropanol Solubility |
| Hemosiderin |
25 ml aliquot of first morning urine specimen. | Negative | Potassium Ferrocyanide-HCL |
| Urine, Qualitative | 10 ml Urine Specimen. No Preservative | Clinitek Atlas Automated Reflectance Spectrophotometer | |
| Hepatitis A (HAV) Antibody, Total IgM Antibody |
2 ml serum 2 ml serum |
Negative Negative |
EIA EIA |
| Hepatitis B (HBV) Core Antibody, Total Core IgM Antibody DNA, Qualitative PCR DNA, Quantitative PCR e Antibody (HBeAb) e Antigen (HBeAg) Surface Antibody (HBsAb), Qualitative Surface Antigen (HBsAg) |
2 ml serum 2 ml serum 1ml frozen EDTA plasma from lavender top tube. Transfer to plastic vial. FREEZE. 1 ml serum. Collect in plain red top tube. Separate and FREEZE. 2 ml serum 2 ml serum 2 ml serum 2 ml serum |
Negative Negative None Detected None Detected None Detected Negative Negative Negative |
EIA EIA PCR PCR EIA EIA EIA EIA |
| Hepatitis C (HCV) Antibody Antibody by Immunoblot Assay (RIBA) RNA by Branched DNA Assay Hep. C RNA QT PCR/TMA Hep. C Genotype |
2 ml serum 1 ml serum. Must be free of hemolysis and lipemia. 1 ml FROZEN serum. Must separate and freeze within one hour after collection or 1ml frozen EDTA plasma. 4ml frozen plasma (draw 2 lavender top tubes) or 4ml frozen serum. 4ml frozen plasma (draw 2 lavender top tubes) or 4ml frozen serum. |
Negative Negative <0.2mEq /ml Negative |
EIA Immunoblot Branched DNA Amplification PCR/TMA PCR, DNA Sequencing |
| Herpes Simplex (HSV) Types 1 and 2, IgG Antibody |
1 ml serum | HSV1: £0.90 HSV2: £0.90 |
EIA |
| Herpes Simplex (HSV) Types 1 and 2, IgM Antibody |
1 ml serum | HSV1: < 1/10 HSV2: < 1/10 |
IFA |
| Herpes Zoster | See Varicella. | ||
| Heterophile Screen | 1 ml serum | Negative | Agglutination |
| Heterophile Titer Serum | 2 ml serum. | Negative | Agglutination |
| Hexosaminidase Studies | See Tay-Sachs | ||
| High Density Lipoprotein | See Cholesterol HDL | ||
| Histamine Blood |
1 green top tube. Transfer plasma to plastic vial and FREEZE. |
9-141 ng/ml | RIA |
| Histoplasma Antibodies Serum |
1 ml Serum Paired sera advisable to direct rising titers. |
Negative | Complement Fixation |
| Histoplasma Antigen | Urine | Negative | |
| HIV(Human Immunodeficiency Virus) HIV 1/2 Antibody Screen with automatic confirmation of equlvocal and Positive results by Westen Blot |
1 red top tube. Must use HIV requisition. Contact laboratory for special forms. |
Non-reactive | Screen: EIA Confirmation: Western Blot |
| HIV-1 Genotyping HIV-1 RNA, PCR 1st Gen HIV-1 RNA, PCR 2nd Gen. HIV-1 RNA, BDNA 3rd Gen. |
2 ml frozen EDTA plasma from Lavender Top tube. Transfer plasma to plastic vial and FREEZE. |
See report <400 copies/ml <2.6 log copies/ml <50 copies/ml <1.7 log copies/ml < 75 copies/ml < 1.9 log copies/ml |
Reverse Transcriptase PCR and DNA sequencing. Reverse transcription- DNA Amplification Branched DNA Signal Amplification |
| HLA-B27 Antigen | 2 full yellow (preferable) or two green top tubes (whole blood) Maintain at room temperature. DO NOT REFRIGERATE. Submit Monday-Thursday only. Include patient’s DOB. Sex and possible diagnosis. Invert tubes several times. |
Negative | Flow Cytometry |
| Homovanillic Acid (HVA) Urine, Quantitative (includes creatinine) |
10 ml Aliquot from well mixed 24 hr. collection. Add 25 ml 6N HCL to container at start of collection. Fecord total volume on requisition. | 0-10 mg/24 hr. Adults: 1.6 – 7.5 mg/24 hr. |
HPLC |
| HTLV I/II Antibody Serum |
2 ml serum | Negative | EIA |
| Human Growth Hormone | See Growth Hormone | ||
| Human Papillomavirus (HPV) DNA Test |
Cervical swab or Frozen Biopsy in ViraPap Transport media. Collect in digene kit | Negative | DNA Hybridization |
| Hydroxyproline Urine, Free (includes creatinine) Urine, Total |
50 ml aliquot of well-mixed 24-hour urine. Add 10 ml HCL to container at start of collection. Refrigerate. Note 24 hour volume on requisition. 50 ml aliquot of well-mixed 24-hour urine. Add 10 ml HCL to container at start of collection. Refrigerate. Note 24 hour volume on requisition. |
< 1.6 ug/ml (for random) Males: 9-73 mg/24 hr. Female: 7-49 mg/24 hr. |
Spectrophotometry Spectrophotometry |
| Imipramine and Desipramine Serum |
3 ml serum. Do not collect in SST. | Therapeutic Range: 150-300 mcg/l |
HPLC |
| Immune Complex (Raji Cells) | 1 ml serum. Collect in plain red top tube. Separate and FREEZE immediately in plastic vial. |
<24 ug/ml - Negative >=24 ug/ml - Positive |
EIA |
| Immunofixation Serum Urine |
2 ml serum. Please provide age and diagnosis. 200 ml aliquot of well mixed 24 hour urine collection Note 24 hour volume on requisition. Please provide age and diagnosis. |
Report includes electrophoretic pattern, interpretation, Total Protein, IgG, IgA, and IgM. Negative |
Nephelometry IEF |
| Immunoglobulin A (IgA) Serum. Quantitative |
1 ml serum | 70-312 mg/dl | Nephelometry |
| Immunoglobulin D (IgD) Serum. Quantitative |
1 ml serum | 0-14 mg/dl | RID |
| Immunoglobulin E (IgE) Serum. Quantitative |
3 ml serum | <120 IU/ml | EIA |
| Immunoglobulin G (IgG) Serum. Quantitative CSF, Quantitative |
1 ml serum 2 ml CSF. FREEZE in plastic vial |
609-1349 mg/dl 0.5-6.1 mg/dl |
Nephelometry Nephelometry |
| Immunoglobulin G (IgG) Subclasses |
3 ml serum FREEZE in plastic vial. | See report | RID |
| Immunoglobulin M (IgM) Serum. Quantitative |
1 ml serum. | 56-352 mg/dl | Nephelometry |
| Infectious Mononucleosis | See Heterophile Titer. | ||
| Influenza A/B Antigen |
Nasopharynx swab in viral transport | Negative | FIA |
| Influenza A+B | Nasopharyngeal Swab in viral transport | Negative | FIA |
| Influenza Antibody (Types A&B) serum |
2 ml serum. Paired sera advisable to detect rising titers. | <1:8 | Complement Fixation |
| Insulin Antibody Serum |
2 ml fasting serum. | None Detected | Radiometric |
| Insulin Serum |
2 ml fasting serum. FREEZE in plastic vial. | <17.0 mIU/ml | RIA |
| Iron-Total, Iron Binding Capacity And Saturation | 4 ml serum. Avoid hemolysis. | Total Iron: 0D=100 – 250 mg/dl 1D= 50 – 120 mg/dl 13D= 40 – 160 mg/dl Male: 55-200 mg/dl Female: 35-200 mg/dl TIBC: 250-425 mg/dl % Saturation: 20-55% |
Spectrophotometry Timed endpoint |
| Ketones Serum, Qualitative Urine, Quantitative |
2 ml serum 5 ml urine |
Negative Negative |
Colorimetry |
| KOH Preparation | Submit sample in sterile tube | Negative | KOH digestion |
| Lactic Acid (Lactate) Plasma, Quantitative |
3 ml plasma. Draw blood in grey top tube. Collect on ice. Centrifuge, separate plasma |
Venous: 0.5-2.2 mmol/l Arterial: 0.5-1.6 mmol/l |
Enzymatic |
| Lactic Dehydrogenase (LDH) Serum Fluid |
2 ml serum. (SST) 1 ml fluid |
91-180 U/L Variable |
Enzymatic Enzymatic |
| Lactic Dehydrogenase (LDH) Serum Fluid |
2 ml serum. (SST) 1 ml fluid |
91-180 U/L Variable |
Enzymatic Enzymatic |
| Lactic Dehydrogenase Isoenzymes-1 Includes Total LDH |
1 ml serum | 25-70 U/L | EIA |
| Lamotrigine | 1 ml serum. Collect in plain red top tube |
4.0 – 18.0 ug/ml | HPLC |
| LASA (Lipid Associated Sialic Acid) | 2 ml serum. Collect in plain red top tube. |
109-262 mcg/dl | Colorimetry |
| Latex Fixation | See Rheumatoid Factor. | ||
| LDL Cholesterol | See Cholesterol LDL | ||
| Lead Blood Urine |
Whole blood-either 1 brown top tube or 1 lavender top tube. Mix gently by inversion. 25 ml aliquot of well-mixed 24-hour urine. Collect in lead free container containing 20 ml of 6N HCL. Note 24 hour volume on requisition. Random urine acceptable. |
<25 mcg/dl >10 mcg/dl should be investigated in children under 6 yrs. 0-80 mcg/l |
Atomic Absorption/ Graphite Furnace Atomic Absorption |
| Lecithin/Sphingomyelin Ratio | 10 ml amniotic fluid. FREEZE in plastic vial. | See report | TLC |
| Legionella (genus) DFA |
Submit specimen in sterile container. Plus DOH form | Negative | Fluorescent Stain |
| Legionella Antibody Pneumophila Antibody (Legionnaire’s Disease) Serum |
1 ml serum. Collect in plain red top tube. Paired sera advisable to detect rising titers. | < 1:64 | IFA |
| Legionella Antigen | Urine in sterile container. | None Detected | EIA |
| Legionella Pneumophila (Legionnaire’s Disease) Culture |
Submit specimen in sterile container. | Negative | Culture |
| Leptospira Agglutination | 2 ml serum. Collect in plain red top tube. |
Negative | Indirect Hemagglutination |
| Leucine Aminopeptidase Serum |
1 mL serum. Collect in plain red top tube. Do not use SST tube |
8-22 mU/ml at 25?C | Spectrophotometry |
| Light Chain Urine |
See Immunofixation | ||
| Lipase Serum | 2 ml serum | 4-24 U/dl. | Timed Enzymatic Rate |
| Lipids, Stool Qualitative Quantitative |
Random stool specimen. Secure special collection container and instructions from laboratory. SHIP FROZEN. |
Negative 1-7 gm/24 hrs. |
Sudan IV stain Microscopy Gravimetric |
| Lithium serum |
1 ml serum. Collect in plain red top tube. | Therapeutic range: 1.0 – 1.20 | Spectrophotometers Endpoint Rate |
| Liver Function Panel | See Test Profiles | ||
| Low Density Lipoprotein(LDL) | See Cholesterol LDL. | ||
| Lupus Anticoagulant | 1 blue top tube. | Ratio interpretation 1.1or less not detected | Photo optical Clot detection |
| Luteinizing Hormone (LH) Serum |
2 ml serum. State patient’s sex and age on request form. | See Addendum. |
EIA |
| Lyme Disease Antibody | 1 ml serum. | EIA | |
| Lysozyme (Muramidase) Serum |
1 ml serum. (SST) | 4.7-14.5 mcg/ml | Radial Immunodifusion |
| Magnesium Serum |
1 ml serum. (SST) | 0D= 1.5 – 1.9 mg/dl 13D= 1.7 – 2.5 mg/dl |
Timed endpoint Colorimetry |
| Malaria, whole Blood | 1 lavender top tube or thick and thin blood smears. | No Malarial Parasites seen | Microscopic Examination |
| Marijuana Screen Urine |
10 ml random urine. | Negative | EIA |
| Measles Antibody Serum |
1 ml serum. Paired sera advisable to detect rising titers. | Negative | EIA |
| Mebaral, (mephobarbital) | 3 ml serum. Collect in plain red top tube. Do not use SST tube | Therapeutic Range: 15-40 mg/l |
Gas Chromatography |
| Mellaril, (Thioridazine and Mesoridazine) | 5 ml serum. Collect in plain red top tube. Do not use SST tube | Therapeutic Range: 0.2-2.6 mg/l |
Gas Chromatography |
| Meprobamate, (Miltown) | 3 ml serum. Collect in plain red top tube. Do not use SST tube | Therapeutic Range: 5.0-30.0 mg/l |
Gas Chromatography |
| Mercury Blood Urine |
2 ml Heparinized Plasma. Dark blue top trace metal tube. Obtain KIT 493 from lab. Transfer plasma within 2 hours to aluminum-free plastic vial (included in KIT 493). 50 ml aliquot of well-mixed 24 hr. urine to special Acid washed transfer tube. Do not add preservative to 24 hr. urine container. Container must also be Acid washed. Contact CDL for special Acid washed container. Note 24 hr volume on requisition. Random urine also acceptable. |
<10 mcg/dl <20 mcg/l Acceptable range for industrial exposure: <150 mcg/l |
Mass spectrometry Mass spectrometry |
| Mesantoin (Mephenytoin) | 2 ml serum. Collect in plain red top tube. Do not collect in SST | Therapeutic Range: 15-40 mg/l |
HPLC |
| Metanephrines, Fractionated Urine |
25 ml aliquot from a well-mixed, 24 hr. collection. Add 30 ml 6N HCL to container at the start of collection. Record total volume on both the specimen container and the test form | Total Metanephrines: 0.0 – 1.30 mg/24hrs Metanephrine: 0.0 – 0.40 mg/24hrs Normatenephrine: 0.0 – 0.90 mg/24hrs |
HPLC |
| Metanephrines, Fractionated Plasma |
4 ml frozen EDTA plasma from Lavender Top tube. Transfer plasma to plastic vial and FREEZE. | Metanephrine: < 0.90 nmol/l Normetanephrine: < 0.50 nmol/l |
HPLC |
| Methemoglobin | 1 Green top tube (Heparin). Assay must be performed within 4 hours of venipuncture. Maintain sample on ice. |
< 2.0% HGB | Colorimetry |
| Methsuximide | 2 ml serum. Collect in plain red top tube. Do not collect in SST | Therapeutic Range: 10-40 mg/l |
HPLC |
| Methylphenidate | 1 ml serum. Collect in plain red top tube. Separate and FREEZE immediately in plastic vial. | 5 –40 ng/ml | HPLC |
| Mexiletene | 2 ml serum. Collect in plain red top tube. Do not collect in SST | Therapeutic Range: 0.7-2.0 mg/l |
HPLC |
| Micro Hemagglutination Antibodies (MHA-ABS) | 1 ml serum | Non-reactive | IFA |
| Microfilaria | 1 lavender top tube. | Negative | Microscopic Exam |
| Microsporidia Stain | Stool | Negative | Microscopic Exam |
| Mumps Virus Antibody Serum |
1 ml serum. Paired sera advisable to detect rising titers. | Negative | EIA |
| Mycoplasma Pneumoniae Antibody, Serum IGG IGM |
2 ml serum. Paired sera advisable. | 11 Negative Negative |
EIA |
| Mycoplasma Pneumoniae Isolation | See Culture, Mycoplasma. | ||
| Myoglobin Serum Urine, Qualitative |
2 ml serum 20 ml Random urine |
<60 mcg/l Negative |
Nephelometry Nephelometry |
| Mysoline | See Primidone | ||
| Nortriptyline Serum |
2 ml serum. Collect in plain red top tube. Do not collect in SST | Therapeutic Range: 50-140 mcg/l | HPLC |
| N-Telopeptide (NTx") Serum Urine |
2 ml serum. Collect in plain red top tube. Do not collect in SST 2 ml urine from 2nd morning void. Collect in sterile container. |
Females: 14 – 76 Males: < 86 |
EIA EIA |
| Occult Blood Stool |
Random stool specimen. | Negative | Guiac Paper Test |
| Osmolality Serum Urine |
2 ml Serum. 10 ml Random urine. |
280-305 mOsm/kg 40-1400 mOsm/kg (no range) |
Freezing Point Depression Freezing Point Depression |
| Ova and Parasites Stool |
10 gms. Random stool in 10% formalin. | None seen. | Microscopic Examination |
| Oxalate Urine |
25 ml aliquot of well-mixed 24-hour urine. Add 30 ml 6N HCL to container at start of collection. PH must be <3. Refrigerate. Record total volume on requisition. | 0-40 mg/24 hrs. | Spectrophotometry |
| Pap Smear | See Cytology. | ||
| Parainfluenze Antiboldy (Types 1,2,3) Serum or CSF |
1 ml serum or CSF Paired sera advisable to detect rising titers. | Serum: <8 CSF: <2 |
Complement Fixation |
| Parasite Identification | Specimen in alcohol. (Not stool) | N/A | Microscopic Examination |
| Parathyroid Hormone(PTH) C-TERMINAL/ MIDREGION Includes Total Calcium |
5 ml serum. FREEZE. Please indicate if patient has renal disease. | 50-340 pg/ml Calcium: 8.5-10.6 mg/dl |
RIA |
| Parathyroid Hormone-Intact Serum |
1ml serum. FREEZE in plastic vial. 2 ml EDTA plasma also acceptable | 12 – 72 pg/ml Calcium: 8.5-10.6 mg/dl |
ECLIA Immunochemiluminometric Sandwich Principle |
| Partial Thromboplastin Time, Activated (aPTT) | 1 blue top tube. Tube must be properly filled to avoid dilution error. Mix thoroughly by gentle inversion. |
21-34.4 seconds | Photo-optical Clot Detection |
| Pathology,Surgical | See Tissue Pathology | ||
| Phenobarbital Serum |
2 ml serum. Do not collect in SST. | Therapeutic Range: 15-40 mg/l |
Particle enhanced turbidmetric Inhibition Immunoassay |
| Phenytoin | 1 ml serum. Do not collect in SST. | Therapeutic Range: 10-20 mg/l |
Particle enhanced turbidmetric Inhibition immunoassay |
| PhosphatidylGlycerol1 ml amniotic fluid | 1 ml amniotic fluid. | Positive indicates fetal maturity | Immunoagglutination |
| Phosphorus Serum Urine |
1 ml serum. 25 ml aliquot of well mixed 24 hr. urine. Add 10 ml 6N HCL at beginning of 24 hr. collection . Record 24 hr volume on requisition. |
Female: 2.6-4.4mg/dl Male: 2.5-6.0mg/dl 0.3-1.3g/24 hrs. |
Colorimetry – Timed endpoint Colorimetry |
| Phosphtidylserine (IgG, IgM) | 1ml plasma from a light blue top tube. | gG- Negative IgM- Negative |
EIA |
| Pinworm | See Enterobius Vermicularis | ||
| Platelet Count Quantitative |
1 lavender top tube. Mix by inversion. |
150-450 k/uL. | Particle Counter |
| Platelet Function Assays | See Coagulation Studies | ||
| Pneumocystis carinii | Sputum in sterile container. | Negative | Immunofluorescence |
| Polio Virus Antibody Types 1,2,3 Serum |
3ml serum. Paired sera advisable to detect rising titers. | <1:4 | Neutralization |
| Porphobilinogen Urine, Quantitative |
15ml aliquot of well mixed 24 hr. urine. Refrigerate while collcting. FREEZE. Wrap container in aluminum foil to protect it from light. Note 24 hr. volume on requisition. | < 2.7 mg/24hr. | Column Chromatography and Spectrophotometry |
| Porphyrins Feces, Quantitative |
100 grams Feces from 48-72 hour collection. Send entire collection FROZEN. | See Addendum. |
HPLC |
| Porphyrins Urine, Quantitative Includes: Coproporphyrins and Uroporphyrins |
15 ml aliquot of 24 hr. urine. Refrigerate during collection. Protect from light by wrapping with aluminum foil. Record 24 hr. volume on requisition. | See Addendum |
HPLC |
| Potassium Fluid Serum Urine |
1 ml fluid (State source) 1 ml serum. (SST) 25 ml aliquot of well mixed 24 hr. urine. Record 24 hr. volume on requisition. |
2.0-5.0 mmol/l 3.5-5.3 mmol/l 25 – 125 mmol/24 hr. |
Ion Selective Electrode Ion Selective Electrode Ion Selective Electrode |
| Pregnancy Test Serum |
See Chorionic Gonadotropin, Urine, Qualitative | ||
| Primidone Serum |
2ml serum. Collect in plain red top tube. Do not collect in SST. | 5-12 mcg/l | EIA |
| Procainamide (Pronestyl) and NAPA Serum |
5ml serum. Collect in plain red top tube. Do not collect in SST. |
4-10 mcg/l | FPIA |
| Progesterone Serum |
4 ml serum. | See Addendum |
RIA |
| Prolactin Serum |
3 ml serum. | F = 3.4 – 24.4 M= 4.1 – 18.4 |
ECLIA Sandwich Principle |
| Prostate Specific Antigen Serum (PSA) |
1 ml serum. | 0-2.5 ng/ml | ECLIA Sandwich Principle |
| Protein C, Activity | One full blue top tube. Separate plasma and FREEZE in plastic vial. | 60-180% | Chromogenic Detection. Photo Optical |
| Protein C, Antigen | One full blue top tube. Separate plasma and FREEZE in plastic vial. | 70-140 % of Normal | EIA |
| Protein Electrophoresis Cerebrospinal fluid Serum Urine |
5 ml CSF 1 ml serum. 50 ml random urine |
See Addendum See Addendum total protein: negative |
Electrophoresis Electrophoresis Electrophoresis |
| Protein S, Activity | One full blue top tube. Separate plasma and FREEZE in plastic vial. | Male: 70-150% Female: 60-140% |
Chromogenic Photo Optical |
| Protein S, Antigen | One full blue top tube. Separate plasma and FREEZE in plastic vial. | 70-140 % of Normal | EIA |
| Protein, Total Fluid Cerebrospinal Fluid Serum Urine |
2 ml fluid. (State source.) 2ml CSF. 1 ml serum. 100 ml aliquot of well mixed 24 hr. urine. Record 24 hr. volume on requisition. |
0-6 gm/dl (No range) 15-45 mg/dl Female: 6.7-8.6 gm/dl Male: 6.2-8.1gm/dl Female= 50-100 mg/24hrs Male= 20-150 mg/24hrs |
Colorimetry biuret Colorimetry Colorimetry Colorimetry |
| Prothrombin Time (PT) | 1blue top tube. Tube must be properly filled to avoid dilution error. Mix gently by inversion 5 or 6 times. | 11-13 seconds | Photo-optical Clot Detection |
| Pseudocholinesterase | 2 ml EDTA plasma. (Lavender top tube). |
3200-6600 U/L | Spectrophotometry |
| PTH | See Parathyroid Hormone. | ||
| Pyruvate Blood |
5 ml protein free filtrate. Collect specimen in a gray top tube. Mix well. Immediately add 4 ml whole blood to 4 ml of 7% perchloric acid and mix. Let stand in the refrigerator for 5-10 minutes to ensure complete precipitation of protein. Centrifuge and submit clear supernatant in plastic vial. | 0.3-0.9 mg/dl | Spectrophotometry |
| Quinidine Serum |
2ml serum from plain red top tube only. Do not collect in SST. | 2.3-5.0 mcg/ml | FPIA |
| RAST | See allergy testing. | ||
| Renin Plasma |
One Blue top tube. Separate plasma and FREEZE in plastic vial. | See Addendum. |
Chemiluminescence |
| Reptilase Time | 1 Blue Top Tube | <23 seconds | Tilt Tube |
| Respiratory Syncytial Virus (RSV) Direct Antigen | Nasopharygeal swab placed in M-4 transport medium. Indicate source on requisition form and on specimen container. | Not Detected | EIA |
| Reticulocyte Cell Count | 1 lavender top tube. Mix gently by inversion. |
0.1-2.5% | Microscopic Examination and Flow Cytometry |
| Rh Phenotype | See ABO Group and Rh Type. | ||
| Rheumatoid Factor Serum |
2 ml serum. | < 20 IU | EIA |
| Ristocetin | One 5 ml blue top tube. Mix. Separate plasma and FREEZE in plastic vial. | 42-200 %Normal | Platelet Agg. |
| Rotavirus | Stool in plain sterile container | negative | ELFA |
| Rubella Antibody Screen Titer |
2 ml serum. 2 ml serum. Paired sera advisable to detect rising titers. |
Immune: >15 IU Equivocal: 10-15 IU NOT Immune: <10 IU |
EIA EIA |
| Rubeola | See Measles | ||
| Saccharomyces Cerevisiae-IgG | 3 ml serum. Do not collect in SST. | Negative: £ 20 Units Equivocal: 20.1- 29.9 Units Positive: >= 30 Units |
|
| Salicylates Serum |
3 ml serum. Do not collect in SST. | 4-100 mg/dl (No range) | Timed endpoint |
| Sedimentation Rate, Erythrocyte | 1 lavender top tube. Mix by gentle inversion. |
Female: 0-20 mm/hr. Male: 0-15 mm/hr. |
Westergreen Excyte Automated |
| Semen Analysis Complete |
Collect entire specimen in clean jar or test tube. Must reach lab within 3 hours of collection. Note collection time and date on requisition. Maintain specimen at 37?C. | See Addendum. |
Microscopic and Macroscopic Examination |
| Serotonin Blood, Quantitative Urine, Qualitative |
One 5 mL. EDTA/Ascorbic Acid whole blood. FREEZE in plastic vial. (Speckled red/yellow top tube) See 5 Hydroxyindoleacetic Acid |
Adult: 50-175 ng/ml | HPLC |
| Sex Chromatin | See Barr Bodies, Buccal Smear. | ||
| SGOT | See Aspartate Amino Transferase | Enzymatic | |
| SGPT | See Alanine Amino Transferase. | Enzymatic | |
| Sickle Cell Screen Blood |
1 lavender top tube. Mix by gentle inversion. |
Negative | Solubility |
| Sirolimus (Rapamycin) | 2 ml whole blood from a lavender top tube (EDTA). | 3.0-18.0 ng/ml | Liquid Chromatography/ Tandem Mass Spectrometry |
| Sjogren’s Antibody | See Anti-ENA Panel | Immunoassay | |
| Sodium Fluid Serum Urine, 24 hr. Urine, Random |
2 ml fluid. 1ml serum. (SST) 20 ml aliquot of well mixed 24 hr. urine. Record total volume on requisition. 20 ml random urine collection. |
Not Established 135-145 mmol/l 43-217mmol/24 hr. Not Established |
Ion Selective Electrode Ion Selective Electrode Ion Selective Electrode Ion Selective Electrode |
| Somatomedin C (Insulin-Like Growth Factor-I) |
3 ml frozen serum. Do not collect in SST. Red Top Tube Only |
See Addendum. |
Chemiluminescence |
| Specific Gravity Fluid |
1 ml fluid. (Specify source) | Variable | Refractometry |
| Sperm Count | See Semen Analysis | ||
| Stain, Esterases (Specific and non-specific) | Blood smear or bone marrow smear. Label slide with patient’s name | See individual Report. | Histochemical stain, microscopy |
| Stain, Periodic Acid Schiff (PAS | Blood smear or bone marrow smear. Label slide with patient’s name. | See individual Report | Histochemical stain, microscopy |
| Stain, Peroxidase | Blood smear or bone marrow smear. Label slide with patient’s name. | See individual Report. | Histochemical stain, microscopy |
| Stain, Sudan Black B | Blood smear or bone marrow smear. Label slide with patient’s name. | See individual Report. | Histochemical stain, microscopy |
| Stain, Tartrate Resistant Acid Phosphatase | Blood smear or bone marrow smear. Label slide with patient’s name. | See individual Report. | Histochemical stain, microscopy |
| Stain, TdT | Blood smear or bone marrow smear. Label slide with patient’s name. | See individual Report. | Immunofluorescence |
| Stone Analysis | See Calculus Analysis. | ||
| Streptozyme Screen | 2 ml serum (SST) | Negative | Immunoassay |
| Synovial Fluid Analysis Includes WBC count, differential crystal analysis, culture, smear, and uric acid |
1 ml fluid in sterile tube, plus 5 ml fluid in red top tube and 2 ml fluid in lavender top tube. Mix by inversion | See Addendum. |
Microscopic Examination |
| Syphilis Serology (RPR) | 1 ml serum. | Non-reactive | Rapid Plasma Reagin Test |
| T and B Cell Enumeration | 1 lavender top tube plus 1 yellow top tube. Ship at room temperature. | Report includes Interpretation. | Flow Cytometry |
| T3 | See Triiodothyronine. | ||
| T4 | See Thyroxine. | ||
| Tay-Sachs Carrier Screen, Non-pregnant Serum |
5 ml serum. Do not collect blood in SST tube. Place on ice immediately after drawing. Separate serum. FREEZE in plastic vial. | See report. | Enzymatic Fluorometry |
| Tay-Sachs Carrier Screen, Pregnant Platelets |
5 blue top tubes. Invert gently 5 times. Refrigerate. Do not centrifuge. | See report. | Enzymatic Fluorometry |
| Tegretol | See Carbamazepine | ||
| Testosterone Free/Weakly Bound And Total |
5 ml serum. Specify patient’s age and sex. |
Males Total: 260-1000 ng/dl Free: 50-210 pg/ml Percent Free testosterone: 1.0-2.7 Females Total: 15-70 ng/l. Free: 1.0-8.5pg/ml % Free Testosterone: 0.5-1.8% |
RIA |
| Theophylline Serum |
1 ml serum. Do not collect in SST. |
Therapeutic Range: 10-20 mg/dl |
Particle enhanced turbidimetric immunoassay |
| Thorazine (Chlorpromazine) Serum |
4 ml serum. Do not use SST. Protect from light by wrapping in aluminum foil. | Therapeutic Range: 30-350mg/l |
Gas Chromatography |
| Thrombin Time | 1 blue top tube. Fill to avoid dilution error. Mix gently by inversion. | 9.4-13.0 seconds. | Photo-optical Clot Detection |
| Thyroglobulin | 2 ml serum | 0-42mg/ml | Chemiluminescence |
| Thyroglobulin Ab. | 2 ml serum | Negative | Chemiluminescence |
| Thyroid Stimulating Hormone | 3 ml serum | 0.27 – 4.2mIU/ml | ECLIA Sandwich Principle |
| Thyroxin Free (Free T4) Serum |
2 ml serum | 0.93 – 1.7ng/dl | ECLIA Competition Principle |
| Thyroxine Binding Globulin (TBG) | 1 ml serum | 16-34 mg/ml | RIA |
| Thyroxine, T4 | 2 ml serum | 5.1-11.1mg/dl | ECLIA Competition Principle |
| Tissue Pathology | Submit specimen in buffered formalin solution. Submit Bone Marrow in Bouin’s Solution. | ||
| Tissue Transglutaminase Ab. IgA | 2 ml serum. Do not collect in SST. | Negative: <20 Units Weak Positive: 20-30 Units Mod – Strong Pos: >30 Units |
EIA |
| Tobramycin Serum |
2 ml serum. Do not collect in SST. | Trough: 1-2 mg/ml Peak: (No range) |
Particle enhanced turbidimetric inhibition immunoassay |
| Toxoplasma Antibodies | 1 ml serum. | <10 IU | EIA |
| Transferrin Serum |
2 ml serum | 215-380 mg/dl | Spectrophotometry - endpoint |
| Treponemal Antibody Test Syphilis |
See Fluorescent Treponemal Antibodies FTA. | ||
| Trichomonas Screen Urine |
10 ml random urine. | Negative | Microscopic Examination |
| Triglycerides Serum |
3 ml serum (SST) | Ages 0D= 20 –150 mg/dl 29D= 46 – 200 mg/dl 39D= 46 – 236 mg/dl |
Enzymatic – Timed endpoint |
| Triiodothyronine (T3) Serum |
3 ml serum. | 0.8-2.0 ng/ml | ECLIA – Competition Principle |
| Urea Nitrogen Serum Urine, 24hr Urine, Random |
1 ml serum. (SST) 10 ml aliquot of well-mixed 24-hour collection. Refrigerate during collection. Note 24-hour volume on requisition. 10 ml random urine |
5-25 mg/dl 5.0-20.0g/24 hrs. (No range for random urine) |
Enzymatic-Electrode Enzymatic-Electrode Enzymatic-Electrode |
| Uric Acid Serum Urine Joint Fluid Crystals |
1 ml serum. (SST) 30 ml aliquot of well-mixed 24-hour collection. Add 10 ml 5N NaOH at beginning of collection. Store at room temperature. Record 24-hour volume on requisition. See Synovial Fluid Analysis. See Synovial Fluid Analysis. |
Female: 2.4-6.9 mg/dl Male: 2.5-8.4 mg/dl 250-750 mg/24 hr. |
Colorimetry – Timed endpoint Colorimetry |
| Urinalysis Complete Without Microscopy Includes: pH Specific gravity, ketones, Protein glucose, blood, And bilirubin |
10 ml aliquot of first morning urine. 10 ml aliquot of first morning urine. |
See Addendum See Addendum |
Microscopic Red Compensator Reflectance Spectophotometer Refractometry |
| Urine Screen (Bacteriuria) |
See Culture, Urine. | ||
| Uroporphyrins | See Porphyrins. | ||
| Valium | See Diazepam. | ||
| Valproic Acid (Depakene) Blood |
2 ml serum. Do not collect in SST. | Therapeutic Range: 50-100 mg/ml |
FPIA |
| Vanilmandelic Acid (VMA) Urine. |
75 ml aliquot of well-mixed 24-hour urine. Add 30 ml 6N HCL. Record 24-hour volume on requisition. | 2.0-10.0-mg/24 hrs. | Spectrophotometry |
| Varicella Antibody Serum (Immune Status) |
1 ml serum. | Immune>0.9 E.U. Not Immune<0.9 E.U. |
EIA |
| Vasoactive Intestinal Polypeptide (VIP) Plasma |
3 ml EDTA plasma. Draw specimen in lavender top tube. Mix gently and chill in ice bath immediately. Separate plasma into 2 plastic vials. FREEZE. | 0-50 pg/ml | RIA |
| Vasopressin | See Anti-Diuretic Hormone | ||
| VDRL Cerebrospinal Fluid |
2 ml CSF. | Non Reactive | Agglutination |
| Viscosity Serum |
4 ml serum. | 1.4-1.8 Rel. to H2O | Viscometry |
| Vitamin A Serum |
2 ml serum. FREEZE in plastic vial. Wrap in aluminum foil to protect it from light. | 30-80 mg/dl | HPLC |
| Vitamin B1 (Thiamine) Blood |
EDTA whole blood frozen (Lavender top tube). Protect from light by wrapping in aluminum foil. | 87-280ng/ml | HPLC |
| Vitamin B12 Serum |
2 ml serum. Fasting specimen preferred. | 200 – 835 pg/ml | ECLIA – Electro Chemiluminescence Immunoassay Competition Principle |
| Vitamin B6 (Pyridoxal-6-Phosphate) Plasma |
3 ml EDTA plasma (Lavender top Tube). FREEZE. Protect from light by wrapping in aluminum foil. | 3.3-26.0ng/ml | HPLC |
| Vitamin C (Ascorbic Acid) Serum |
2 ml serum. FREEZE in plastic vial. Protect from light by wrapping in aluminum foil. | 0.2-2.0 mg/dl | Spectrophotometry |
| Vitamin D (1, 25 dihydroxy) Serum |
5 ml serum. FREEZE. | 19-67pg/ml | RIA |
| Vitamin D (25 Hydroxy) Serum |
2 ml serum. FREEZE | 9-52ng/ml | Competitive Protein Binding |
| Vitamin E (Tocopherol) | 3 ml serum. FREEZE in plastic vial. Protect from light by wrapping in aluminum foil. | Alpha Tocopherol: 5.7-19.9mg/l Beta-Gamma Tocopherol: £4.3mg/l |
HPLC |
| Vitamin K1 | 3ml frozen plasma (Green Top Tube). Protect from light by wrapping in aluminum foil. | 80-1160pg/ml | HPLC |
| Xylose Absorption Test Urine Blood |
15 ml urine-Plastic container. Aliquot from a well-mixed, 5-hour collection. Refrigerate during collection and prior to delivery to laboratory. If specimen is to be stored for some time, add NaF (10 mg per mL of urine) as a preservative. Record total volume for 5-hour collection, and the amount of D-Xylose administered on both the specimen container and the test request form. Patient must be fasting before and during this test. 1 full gray top tube. Mix gently by inversion. Collect two hours after administering D-Xylose. |
See Addendum See Addendum |
Spectrophotometry Spectrophotometry |
| Zarontin | See Ethosuximide. | ||
| Zinc Plasma |
Use Kit 493 1 royal blue top tube. Mix gently by inversion. Separate plasma from cells within 2 hrs. | 60-130 mg/dl | Atomic Absorption |